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What is the difference between direct and indirect ELISA?
What is the difference between direct and indirect ELISA?
Direct ELISA uses a primary labeled antibody that reacts directly with the antigen, while indirect ELISA utilizes a primary unlabeled antibody in conjunction with a labeled secondary antibody.
What are the key components involved in the ELISA test?
What are the key components involved in the ELISA test?
Antigen and antibody
What are the applications of ELISA in detecting biological molecules?
What are the applications of ELISA in detecting biological molecules?
To measure antibody levels, detect hormonal changes, and detect circulatory inflammatory markers.
What are some disadvantages of ELISA?
What are some disadvantages of ELISA?
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What is the role of the secondary antibody in indirect ELISA?
What is the role of the secondary antibody in indirect ELISA?
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