ELISA Test Principles and Applications

Direct ELISA uses a primary labeled antibody that reacts directly with the antigen, while indirect ELISA utilizes a primary unlabeled antibody in conjunction with a labeled secondary antibody.

Antigen and antibody

To measure antibody levels, detect hormonal changes, and detect circulatory inflammatory markers.

<p>Measurement of enzyme activity can be complex, enzyme activity may be affected by plasma constituents, kits are expensive, specific to particular antigen, and can lead to false positive/negative results.</p> Signup and view all the answers

<p>The secondary antibody has specificity for the primary antibody and is used to detect small molecules like T₃, T₄, and Progesterone.</p> Signup and view all the answers