5 Questions
What is the difference between direct and indirect ELISA?
Direct ELISA uses a primary labeled antibody that reacts directly with the antigen, while indirect ELISA utilizes a primary unlabeled antibody in conjunction with a labeled secondary antibody.
What are the key components involved in the ELISA test?
Antigen and antibody
What are the applications of ELISA in detecting biological molecules?
To measure antibody levels, detect hormonal changes, and detect circulatory inflammatory markers.
What are some disadvantages of ELISA?
Measurement of enzyme activity can be complex, enzyme activity may be affected by plasma constituents, kits are expensive, specific to particular antigen, and can lead to false positive/negative results.
What is the role of the secondary antibody in indirect ELISA?
The secondary antibody has specificity for the primary antibody and is used to detect small molecules like T₃, T₄, and Progesterone.
Learn about the principles and applications of the Enzyme-Linked Immunosorbent Assay (ELISA) test in this lecture. Understand the key concepts such as antigens, antibodies, and the different types of ELISA tests. Gain insights into the non-competitive ELISA and the direct and indirect ELISA methods.
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