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ELISA Steps 6-8

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Questions and Answers

Which of the following is the primary function of antibodies in immunoassay techniques?

To diagnose and detect diseases
To identify the presence of specific proteins
To label antigens with fluorescent dyes
To cause a color change in the presence of a specific antigen (correct)

What is the purpose of using monoclonal antibodies in the diagnosis and detection of disease?

Monoclonal antibodies can bind to a single specific antigen with high affinity
Monoclonal antibodies can be produced in large quantities from hybridomas
Monoclonal antibodies can be used in the ELISA technique to identify antigens
All of the above (correct)

What is the main advantage of using monoclonal antibodies over polyclonal antibodies?

Monoclonal antibodies can be produced in larger quantities
Monoclonal antibodies have fewer non-specific interactions
Monoclonal antibodies have higher affinity for their target antigen
Monoclonal antibodies recognize a single epitope on the antigen (correct)

How are hybridomas formed to produce monoclonal antibodies?

By fusing a B lymphocyte with a myeloma cell using polyethylene glycol (PEG) (C) Signup and view all the answers

What is the primary purpose of using the ELISA technique in antibody-based assays?

To identify the presence of specific antigens in a sample (D) Signup and view all the answers

What is the role of myeloma cells in the production of monoclonal antibodies?

Myeloma cells provide the immortality and continuous antibody production needed for hybridoma formation (B) Signup and view all the answers

How do hybridomas differ from normal B lymphocytes in their ability to produce antibodies?

Hybridomas can only produce one specific antibody, while B lymphocytes can produce a variety of antibodies (B) Signup and view all the answers

Why is polyethylene glycol (PEG) used in the production of hybridomas?

PEG is used to fuse the B lymphocyte and myeloma cell to create the immortal hybridoma (B) Signup and view all the answers

What is the primary function of the selective media used in hybridoma production?

To screen and identify the specific hybridoma cells producing the desired antibody (D) Signup and view all the answers

How are monoclonal antibodies extracted and purified from hybridoma cultures?

By batch culturing the secreting hybridomas in fermenters and extracting the pure antibody (A) Signup and view all the answers

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