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Questions and Answers
What is the setup in a sandwich Dot-ELISA?
What is the setup in a sandwich Dot-ELISA?
- Antibodies immobilized on a glass slide
- Antigen directly mixed with detecting antibody
- Two different antigens reacting with one antibody
- Antigen between two antibodies on a nitrocellulose membrane (correct)
How is the capturing antibody immobilized in Dot-ELISA?
How is the capturing antibody immobilized in Dot-ELISA?
- Onto a nitrocellulose membrane (correct)
- Onto a plastic surface
- Onto a glass slide
- Onto a metal plate
What happens when antigen in the test sample reacts with the second enzyme-linked antibody in Dot-ELISA?
What happens when antigen in the test sample reacts with the second enzyme-linked antibody in Dot-ELISA?
- The antigen disintegrates
- The antigen becomes soluble
- A coloured, insoluble product is formed (correct)
- The antigen loses its reactivity
What is avidin derived from in Avidin Biotin ELISA (AB-ELISA)?
What is avidin derived from in Avidin Biotin ELISA (AB-ELISA)?
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What is biotin in Avidin Biotin ELISA (AB-ELISA)?
What is biotin in Avidin Biotin ELISA (AB-ELISA)?
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How many biotin-binding sites per molecule do avidin/streptavidin proteins have?
How many biotin-binding sites per molecule do avidin/streptavidin proteins have?
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'In sandwich Dot-ELISA, the antigen is sandwiched directly between how many antibodies?'
'In sandwich Dot-ELISA, the antigen is sandwiched directly between how many antibodies?'
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'What kind of substrate is used in Dot-ELISA to detect enzyme-linked antibody binding?'
'What kind of substrate is used in Dot-ELISA to detect enzyme-linked antibody binding?'
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