8 Questions
What is the setup in a sandwich Dot-ELISA?
Antigen between two antibodies on a nitrocellulose membrane
How is the capturing antibody immobilized in Dot-ELISA?
Onto a nitrocellulose membrane
What happens when antigen in the test sample reacts with the second enzyme-linked antibody in Dot-ELISA?
A coloured, insoluble product is formed
What is avidin derived from in Avidin Biotin ELISA (AB-ELISA)?
Avians and amphibians
What is biotin in Avidin Biotin ELISA (AB-ELISA)?
A small vitamin molecule
How many biotin-binding sites per molecule do avidin/streptavidin proteins have?
Four
'In sandwich Dot-ELISA, the antigen is sandwiched directly between how many antibodies?'
Two antibodies
'What kind of substrate is used in Dot-ELISA to detect enzyme-linked antibody binding?'
Chromogenic substrate
Test your knowledge about the advantages of Competitive ELISA, a technique that offers high specificity, sensitivity, and flexibility in detecting antibodies. Learn about its suitability for complex samples and the use of two antibodies for capturing and detecting antigens.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
