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Competitive ELISA Advantages Quiz

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Questions and Answers

What is the setup in a sandwich Dot-ELISA?

Antibodies immobilized on a glass slide

Antigen directly mixed with detecting antibody

Two different antigens reacting with one antibody

Antigen between two antibodies on a nitrocellulose membrane (correct)

How is the capturing antibody immobilized in Dot-ELISA?

Onto a nitrocellulose membrane (correct)

Onto a plastic surface

Onto a glass slide

Onto a metal plate

What happens when antigen in the test sample reacts with the second enzyme-linked antibody in Dot-ELISA?

The antigen disintegrates

The antigen becomes soluble

A coloured, insoluble product is formed (correct)

The antigen loses its reactivity

What is avidin derived from in Avidin Biotin ELISA (AB-ELISA)?

Avians and amphibians Signup and view all the answers

What is biotin in Avidin Biotin ELISA (AB-ELISA)?

A small vitamin molecule Signup and view all the answers

How many biotin-binding sites per molecule do avidin/streptavidin proteins have?

Four Signup and view all the answers

'In sandwich Dot-ELISA, the antigen is sandwiched directly between how many antibodies?'

Two antibodies Signup and view all the answers

'What kind of substrate is used in Dot-ELISA to detect enzyme-linked antibody binding?'

Chromogenic substrate Signup and view all the answers