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Sandwich ELISA: Antibodies and Antigen Detection

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Sandwich ELISA: Antibodies and Antigen Detection

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Questions and Answers

In a Sandwich ELISA, how many antibodies are typically used?

Three antibodies

Four antibodies

One antibody

Two antibodies (correct)

What type of antibodies can be used for both capture and detection in a Sandwich ELISA?

Monoclonal antibodies only

Either monoclonal or polyclonal antibodies (correct)

Polyclonal antibodies only

Antibodies against the same epitope

What is the purpose of drawing a standard curve in a Sandwich ELISA?

To calibrate the ELISA reader

To quantify the test antigen by extrapolation (correct)

To confirm the presence of epitopes on the antigen

To determine the type of antibodies to use

What is a key advantage of using the sandwich ELISA format for detecting viral pathogens?

High specificity due to the use of two antibodies Signup and view all the answers

How does the sandwich ELISA format avoid the need for purifying antigen from complex mixtures?

By using two antibodies for capturing and detecting the antigen Signup and view all the answers

What is a disadvantage of the sandwich ELISA format compared to other methods?

Involves an additional incubation step in the procedure Signup and view all the answers

In a competitive ELISA, how does the signal vary in relation to the concentration of the target analyte?

Decreases with higher analyte concentration Signup and view all the answers

What effect does a high concentration of the target analyte have on the reference signal in a competitive ELISA?

Diminishes the reference signal Signup and view all the answers

What is the main purpose of conducting a competitive ELISA?

To quantify interference of analyte with labeled antibody Signup and view all the answers

Sandwich ELISA: Antibodies and Antigen Detection

Choose a study mode

Podcast

Questions and Answers

In a Sandwich ELISA, how many antibodies are typically used?

What type of antibodies can be used for both capture and detection in a Sandwich ELISA?

What is the purpose of drawing a standard curve in a Sandwich ELISA?

What is a key advantage of using the sandwich ELISA format for detecting viral pathogens?

How does the sandwich ELISA format avoid the need for purifying antigen from complex mixtures?

What is a disadvantage of the sandwich ELISA format compared to other methods?

In a competitive ELISA, how does the signal vary in relation to the concentration of the target analyte?

What effect does a high concentration of the target analyte have on the reference signal in a competitive ELISA?

What is the main purpose of conducting a competitive ELISA?

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