Podcast
Questions and Answers
In a Sandwich ELISA, how many antibodies are typically used?
In a Sandwich ELISA, how many antibodies are typically used?
- Three antibodies
- Four antibodies
- One antibody
- Two antibodies (correct)
What type of antibodies can be used for both capture and detection in a Sandwich ELISA?
What type of antibodies can be used for both capture and detection in a Sandwich ELISA?
- Monoclonal antibodies only
- Either monoclonal or polyclonal antibodies (correct)
- Polyclonal antibodies only
- Antibodies against the same epitope
What is the purpose of drawing a standard curve in a Sandwich ELISA?
What is the purpose of drawing a standard curve in a Sandwich ELISA?
- To calibrate the ELISA reader
- To quantify the test antigen by extrapolation (correct)
- To confirm the presence of epitopes on the antigen
- To determine the type of antibodies to use
What is a key advantage of using the sandwich ELISA format for detecting viral pathogens?
What is a key advantage of using the sandwich ELISA format for detecting viral pathogens?
How does the sandwich ELISA format avoid the need for purifying antigen from complex mixtures?
How does the sandwich ELISA format avoid the need for purifying antigen from complex mixtures?
What is a disadvantage of the sandwich ELISA format compared to other methods?
What is a disadvantage of the sandwich ELISA format compared to other methods?
In a competitive ELISA, how does the signal vary in relation to the concentration of the target analyte?
In a competitive ELISA, how does the signal vary in relation to the concentration of the target analyte?
What effect does a high concentration of the target analyte have on the reference signal in a competitive ELISA?
What effect does a high concentration of the target analyte have on the reference signal in a competitive ELISA?
What is the main purpose of conducting a competitive ELISA?
What is the main purpose of conducting a competitive ELISA?
