9 Questions
In a Sandwich ELISA, how many antibodies are typically used?
Two antibodies
What type of antibodies can be used for both capture and detection in a Sandwich ELISA?
Either monoclonal or polyclonal antibodies
What is the purpose of drawing a standard curve in a Sandwich ELISA?
To quantify the test antigen by extrapolation
What is a key advantage of using the sandwich ELISA format for detecting viral pathogens?
High specificity due to the use of two antibodies
How does the sandwich ELISA format avoid the need for purifying antigen from complex mixtures?
By using two antibodies for capturing and detecting the antigen
What is a disadvantage of the sandwich ELISA format compared to other methods?
Involves an additional incubation step in the procedure
In a competitive ELISA, how does the signal vary in relation to the concentration of the target analyte?
Decreases with higher analyte concentration
What effect does a high concentration of the target analyte have on the reference signal in a competitive ELISA?
Diminishes the reference signal
What is the main purpose of conducting a competitive ELISA?
To quantify interference of analyte with labeled antibody
Learn about the sandwich ELISA assay which utilizes two different antibodies (capture and detection) to detect antigens. Understand the importance of using antibodies with different epitopes for accurate measurements, and the role of monoclonal and polyclonal antibodies in improving test sensitivity and specificity.
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