Analgesic Effect of Diclofenac Sodium Experiment
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Questions and Answers

What is the purpose of the experiment involving Diclofenac Sodium?

  • To induce writhing in mice using saline
  • To prepare the acetic acid solution for future experiments
  • To observe the analgesic effect in mice induced by acetic acid (correct)
  • To measure the weight of the mice before injection
  • What method is used to administer Diclofenac Sodium in the experiment?

  • Subcutaneous injection
  • Oral ingestion
  • Intraperitoneal injection (correct)
  • Intramuscular injection
  • Why should acetic acid solution be prepared just before its use?

  • To increase its effectiveness
  • To avoid contamination
  • To ensure it is at room temperature
  • Because it is a volatile solution (correct)
  • What is the significance of measuring the number of writhing mice in the experiment?

    <p>To calculate the analgesic percentage of the drug</p> Signup and view all the answers

    Which factor is mentioned as affecting the writhing behavior of the mice?

    <p>The temperature of the laboratory</p> Signup and view all the answers

    In the experiment, how are the mice grouped before injections?

    <p>In two groups, each containing two mice</p> Signup and view all the answers

    What is the weight range of the mice selected for the experiment?

    <p>20-25 grams</p> Signup and view all the answers

    What calculation is performed at the end of the experiment?

    <p>The percentage of analgesia achieved with Diclofenac Sodium</p> Signup and view all the answers

    Study Notes

    Analgesic Effect of Diclofenac Sodium

    • Experimental Purposes:
      • Observe acetic acid-induced writhing in mice.
      • Observe the analgesic effect of Diclofenac Sodium.

    Experimental Principle

    • Pain Models: Physical or chemical stimuli cause pain.
    • Acetic Acid-Induced Writhing: Acetic acid injected intraperitoneally causes abdominal pain (writhing) in mice.
    • Mechanism: Intraperitoneal injection of chemicals like acetic acid stimulates the peritoneum, leading to abdomen constriction, trunk twisting, and hind limb extension.
    • NSAIDs and Analgesia: Nonsteroidal anti-inflammatory drugs (NSAIDs) like Diclofenac Sodium can reduce pain and writhing by affecting the cyclooxygenase (COX) pathway, which reduces prostaglandin (PG) production.

    Experimental Materials

    • Animals: 4 mice, weighing 20-25 grams.
    • Drugs: Saline, 0.6% acetic acid, and Diclofenac Sodium solution.

    Experimental Procedure

    • Grouping: Divide the mice into two groups: control and test.
    • Drug Injection: Administer saline to the control group and Diclofenac Sodium to the test group, based on mouse weight.
    • Acetic Acid Injection: Inject 0.5 ml of 0.6% acetic acid solution intraperitoneally.
    • Observation and Recording: Observe and record the number of writhing mice in each group within 15 minutes after acetic acid injection.

    Results and Analysis

    • Data Collection: Data is collected regarding the number of writhing mice in the control and test groups.
    • Analgesic Percentage Calculation: A formula is used to calculate the analgesic percentage of Diclofenac Sodium.

    Application Notice

    • Acetic Acid Preparation: Acetic acid solution must be freshly prepared before each use.
    • Mouse Selection: Larger mice (20-24 g) are preferred, as low-weight mice may not display significant writhing.
    • Temperature Control: Maintain laboratory temperature at 18-22°C to avoid affecting the writhing response.

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    Description

    This quiz explores the analgesic effect of Diclofenac Sodium through an experiment observing acetic acid-induced writhing in mice. It covers the experimental principles, materials used, and the procedure involved in assessing the pain-relieving properties of this NSAID. Dive into the mechanisms of pain and how NSAIDs function to alleviate it.

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