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Questions and Answers
What is the purpose of the experiment involving Diclofenac Sodium?
What is the purpose of the experiment involving Diclofenac Sodium?
- To induce writhing in mice using saline
- To prepare the acetic acid solution for future experiments
- To observe the analgesic effect in mice induced by acetic acid (correct)
- To measure the weight of the mice before injection
What method is used to administer Diclofenac Sodium in the experiment?
What method is used to administer Diclofenac Sodium in the experiment?
- Subcutaneous injection
- Oral ingestion
- Intraperitoneal injection (correct)
- Intramuscular injection
Why should acetic acid solution be prepared just before its use?
Why should acetic acid solution be prepared just before its use?
- To increase its effectiveness
- To avoid contamination
- To ensure it is at room temperature
- Because it is a volatile solution (correct)
What is the significance of measuring the number of writhing mice in the experiment?
What is the significance of measuring the number of writhing mice in the experiment?
Which factor is mentioned as affecting the writhing behavior of the mice?
Which factor is mentioned as affecting the writhing behavior of the mice?
In the experiment, how are the mice grouped before injections?
In the experiment, how are the mice grouped before injections?
What is the weight range of the mice selected for the experiment?
What is the weight range of the mice selected for the experiment?
What calculation is performed at the end of the experiment?
What calculation is performed at the end of the experiment?
Flashcards
What is an NSAID?
What is an NSAID?
A type of pain relief medication that works by reducing inflammation.
What is Diclofenac Sodium?
What is Diclofenac Sodium?
A specific type of NSAID used to relieve pain and inflammation.
What is 'writhing' in mice?
What is 'writhing' in mice?
A reaction in mice caused by injecting acetic acid. It makes them twist and stretch their bodies, mimicking pain.
What is analgesic effect?
What is analgesic effect?
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What is the purpose of the acetic acid writhing test?
What is the purpose of the acetic acid writhing test?
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How is the analgesic percentage of Diclofenac Sodium calculated?
How is the analgesic percentage of Diclofenac Sodium calculated?
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Why is the room temperature important for the experiment?
Why is the room temperature important for the experiment?
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Why is it important to prepare the drug dose according to the mice's weight?
Why is it important to prepare the drug dose according to the mice's weight?
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Study Notes
Analgesic Effect of Diclofenac Sodium
- Experimental Purposes:
- Observe acetic acid-induced writhing in mice.
- Observe the analgesic effect of Diclofenac Sodium.
Experimental Principle
- Pain Models: Physical or chemical stimuli cause pain.
- Acetic Acid-Induced Writhing: Acetic acid injected intraperitoneally causes abdominal pain (writhing) in mice.
- Mechanism: Intraperitoneal injection of chemicals like acetic acid stimulates the peritoneum, leading to abdomen constriction, trunk twisting, and hind limb extension.
- NSAIDs and Analgesia: Nonsteroidal anti-inflammatory drugs (NSAIDs) like Diclofenac Sodium can reduce pain and writhing by affecting the cyclooxygenase (COX) pathway, which reduces prostaglandin (PG) production.
Experimental Materials
- Animals: 4 mice, weighing 20-25 grams.
- Drugs: Saline, 0.6% acetic acid, and Diclofenac Sodium solution.
Experimental Procedure
- Grouping: Divide the mice into two groups: control and test.
- Drug Injection: Administer saline to the control group and Diclofenac Sodium to the test group, based on mouse weight.
- Acetic Acid Injection: Inject 0.5 ml of 0.6% acetic acid solution intraperitoneally.
- Observation and Recording: Observe and record the number of writhing mice in each group within 15 minutes after acetic acid injection.
Results and Analysis
- Data Collection: Data is collected regarding the number of writhing mice in the control and test groups.
- Analgesic Percentage Calculation: A formula is used to calculate the analgesic percentage of Diclofenac Sodium.
Application Notice
- Acetic Acid Preparation: Acetic acid solution must be freshly prepared before each use.
- Mouse Selection: Larger mice (20-24 g) are preferred, as low-weight mice may not display significant writhing.
- Temperature Control: Maintain laboratory temperature at 18-22°C to avoid affecting the writhing response.
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