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Questions and Answers
The experiment involves observing the analgesic effect of Acetic acid in mice.
The experiment involves observing the analgesic effect of Acetic acid in mice.
False
Diclofenac Sodium is administered to the mice by oral ingestion.
Diclofenac Sodium is administered to the mice by oral ingestion.
False
The control group receives Saline while the test group receives Diclofenac Sodium.
The control group receives Saline while the test group receives Diclofenac Sodium.
True
The writhing behavior of the mice is measured for 30 minutes after drug injection.
The writhing behavior of the mice is measured for 30 minutes after drug injection.
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Mice weighing between 20g and 25g are suitable for this experiment.
Mice weighing between 20g and 25g are suitable for this experiment.
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Acetic acid solution is stable and can be prepared in advance.
Acetic acid solution is stable and can be prepared in advance.
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The temperature in the lab should be maintained between 18 and 22 degrees Celsius.
The temperature in the lab should be maintained between 18 and 22 degrees Celsius.
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The analgesic percentage of Diclofenac Sodium is calculated using the number of non-writhing mice.
The analgesic percentage of Diclofenac Sodium is calculated using the number of non-writhing mice.
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Study Notes
Analgesic Effect of Diclofenac Sodium
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Experimental Purposes:
- Observe writhing induced by acetic acid.
- Observe analgesic effect of Diclofenac Sodium in mice.
Experimental Principle
- Pain Models: Physical or chemical stimulation can induce pain.
- Acetic Acid-Induced Writhing: Intraperitoneal injection of acetic acid induces abdominal pain (writhing) in mice, characterized by abdomen constriction, trunk twisting, and hind limb extension.
- NSAIDs and Analgesia: Nonsteroidal anti-inflammatory drugs (NSAIDs), like Diclofenac Sodium, reduce pain and writhing by inhibiting the production of prostaglandins.
Experimental Material
- Animals: 4 mice weighing 20-25g.
- Drugs: Saline, 0.6% acetic acid, and Diclofenac Sodium solution.
Experimental Procedure
- Grouping: Divide the mice into two groups (control and test group), with 2 mice per group.
- Weighing and Marking: Weigh mice and mark them.
- Drug Injection: Administer saline to the control group and Diclofenac Sodium to the test group, dose adjusted according to mouse weight.
- Acetic Acid Injection: 20 minutes after the drug injection, administer 0.5ml of 0.6% acetic acid per mouse intraperitoneally.
- Observation and Recording: Observe and record the number of writhing mice in each group within 15 minutes after acetic acid injection.
Results and Analysis
- Table 1: Data summarizing the number of writhing mice in the control and test groups will reveal the analgesic effect of Diclofenac Sodium. Percentage of analgesic effect is calculated as (b-B)/A x 100%, where b & B are non-writhing mice from the test and control groups respectively, and A is number of writhing mice in control group.
Application Notice
- Acetic Acid Preparation: Acetic acid solution must be freshly prepared before each use and stored covered to prevent evaporation.
- Mice Selection: Choose mice with appropriate weight (20-24g) for accurate observation of writhing.
- Temperature Control: Maintain a constant laboratory temperature (18-22°C) to minimize environmental influence on the mice's response.
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Description
This quiz explores the analgesic effects of Diclofenac Sodium through experimental observation of writhing induced by acetic acid in mice. Participants will learn about pain models, the role of NSAIDs, and the experimental procedure involved in administering and assessing pain relief. Understand the principles behind analgesia and how it is tested in a laboratory setting.
