Results of Paper Chromatography - PDF

Results The results of the paper chromatography can be seen in Figure 2 to the right. Calculations Use the ruler located at...

Results The results of the paper chromatography can be seen in Figure 2 to the right. Calculations Use the ruler located at the right side of the paper D chromatography to calculate the Rf value for each amino acid in each sample. Show your work below: B F 1 8 21 E 48 28 A E B si FI 61 0 3 D 78 Analysis 73 1. Referring to the procedure, what is the purpose of exposing the dipeptide samples to a 6NAHCl solution? To break up the dipeptide into individual amino acids 2. What is the purpose of spraying the paper with a 0.5% solution of ninhydrin? Allow colors to develop 3. Based on your calculations, what amino acids are in samples 1-3? 1. Arginine and Asparagine 2. Glutamic acid and Leucine 3. Valine and serine 4. Once you have determined the amino acid composition of each sample, you are told that each sample was taken from a specific species of bacteria. The dipeptide samples represent the amino acids that are most important to the bacteria’s survival and reproduction. You are not told which sample represents which bacteria, but you are given the amino acid composition of the medium that each bacteria grows best in. “Medium” is a solution of nutrients that allow bacteria to survive and reproduce in a lab. Use the table below to determine the species of bacteria that each sample represents. © Getting Down With Science 2020 sample sample sample 75