The Microscope PDF

THE MICROSCOPE Imelda R. Romero, Ph.D. Department of Biological Sciences School of Science and Technology THE MICROSCOPE Icon The Microscope Magnifies very small objects Allows the study of tiny objects NOT seen by the naked eyes CENTRO ESCOLAR UNIVERSITY...

THE MICROSCOPE Imelda R. Romero, Ph.D. Department of Biological Sciences School of Science and Technology THE MICROSCOPE Icon The Microscope Magnifies very small objects Allows the study of tiny objects NOT seen by the naked eyes CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 TYPES OF MICROSCOPE TYPES OF MICROSCOPE Simple Microscope Single lens microscope, with only 1 lens Icon 1st one was made by Anton van Leeuwenhoek (1672), with a magnification of 200x - 300x Simple Compound Microscope 2 Sets of lenses Magnifies 100x – 200x Zaccharias Janssen and his son Hans Janssen (1590) made the 1st simple compound microscope CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 TYPES OF MICROSCOPE TYPES OF MICROSCOPE Laboratory Compound Microscope Developed by Robert Hooke (1665) Icon With Oil Lamps and Condensing Lenses for illumination Modern Compound Microscope With more than 1 lens 2 sets of lenses, the eyepiece and objectives Has 3 to 5 objective lenses CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 TYPES OF MICROSCOPE TYPES OF MICROSCOPE Electron Microscope Magnifies object up to 300,000x Icon Do not use lenses, but use electrons to enlarge the image CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 TERMINOLOGIES TERMS TO REMEMBER MAGNIFICATION: ratio of the size of an object seen under the microscope to that of its actual size RESOLVING POWER: ability of the microscope to differentiate 2 close points as separate WORKING DISTANCE: distance between the objective and the objective slide * Working Distance decreases with increasing magnification NUMERICAL APERTURE: ratio of the diameter of the lens to its focal length *Numerical Aperture decreases with decreasing amount of light CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 THE PARTS OF THE MICROSCOPE The 3 Main Parts of the Microscope The Compound Microscope has 3 Main Parts, these are: MECHANICAL PARTS: metal parts of the microscope ILLUMINATING PARTS: parts for the supply of light OPTICAL PARTS: parts for magnification CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 Mechanical Parts of the Microscope 1. Arm 2. Base Connects the upper parts Serves as the “foot” and lower parts of the of the microscope 1 microscope To safely hold the 3 Image 4 microscope 3. Stage 4. Stage Clips Platform where the slide To hold the slide in with the specimen is placed 2 placed CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 Mechanical Parts of the Microscope 5 6 5. Draw Tube 6. Body Tube Holds the eyepiece Allows a considerable 7 distance between the 8 eyepiece and Image objective lens 7. Dusts Shield 8. Revolving Nosepiece Avoid the accumulation of Holds the objective lenses dust in the objective Allows the shifting of the lenses objective lenses CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 Mechanical Parts of the Microscope 9. Coarse Adjustment Knob To bring the objective into focus Large adjustment of focus Image 9 10 10. Fine Adjustment Knob To bring the image into focus Small adjustment of focus CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 Illuminating Parts of the Microscope 1. Mirror / Lamp 2. Diaphragm Mirror: gathers light Regulates the amount Lamp: source of light of light Image 3 3. Condenser 2 Concentrates light to the 1 specimen CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 Optical Parts of the Microscope 1 1. Eyepiece / Ocular Lens on top of the microscope thus, where you peep into to see the specimen Magnifies the SECONDARY IMAGE of the specimen 2 Usually with 10x magnification Image 2. Objective Lenses Mounted into the Revolving Nosepiece above the stage Magnifies the PRIMARY IMAGE of the specimen 3 Types: 1. Low Power Objective 2. High Power Objective 3. Oil Immersion Objective CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 OPTICAL PARTS OF THE MICROSCOPE 3 OBJECTIVE LENSES Low Power High Power Objective (LPO) Objective (HPO) 10x magnification 40x magnification Initial Focusing Shows specific/detailed Shows General Image part of the specimen Oil Immersion Scanning Power IconObjective (OIO) Icon Objective (SPO) 100x magnification 4x magnification For the study of Scan the whole slide microorganisms With the use of cedarwood oil CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 PRESENTATION TITLE LINE THANK YOU! CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018 REFERENCES https://www.slideshare.net/sarathy4/microscope-10905635 www.bgreen.kyschools.us › userfiles › Classes › MicroscopeBasics https://www.pinterest.ph/pin/782570872727493009/ http://www.funsci.com/fun3_en/antoni/vlen.htm https://en.wikipedia.org/wiki/Zacharias_Janssen https://sites.google.com/site/rhookemicroscope/ https://microbiologynotes.com/differences-between-simple-and-compound-microscope/ https://www.scienceprofonline.com/science-image-libr/sci-image-libr-microscope.html https://www.amazon.in/OMAX-Achromatic-Microscope-Objective-Coating/dp/B00FG8EUQQ Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed sit amet magna tristique, hendrerit eros non, vulputate risus. Ut in aliquam leo. CENTRO ESCOLAR UNIVERSITY FOR INTERNAL CIRCULATION ONLY 2018