Plasma Lipoproteins & Lipids PDF

Plasma lipoprotein & lipids | Session 7 Massa Zahdeh, Ruba Jaafreh Role.Transport...

Plasma lipoprotein & lipids | Session 7 Massa Zahdeh, Ruba Jaafreh Role.Transport > - triglycerols and cholesterol in blood Lipoproteins between all tissues in body ~ - Comconents Classes 2 ↳ (Density , Size , Content ofCholesterd) > - - TG > Proteins Lipi dS ↓ protein ↓ ↓ Chylomicrons. 1 Duter surface Innermost surface ↓ X 2. VLDL Exogenous Endogenous. IDL 3 ↓ # Diet Liver 4 LDL. 5 HDL. - Control platform for handling of Lipids Store- Dycerols in hepatocytes Liver - ↳ fets ↳ & Create triacylglycerols (he novo synthesis) N C Produce Bilo from Cholesterd LDL (BAD Cholesterol) HDL (Good Cholesterol) * Carries Cholesterol around body parts * protect against CVDs > - Responsible for excess Shelosterol ~ Liver T Cholesterd If - in blood stream clogging Stimulate Breaks TG-free fatty acids tr (in wells > - Glycerol Blook Vessels Cling to ↓ ↓ Atherosclerosis  Determination of Serum TG patients is important in * HDL hypertrigliceridemia : * 2 * Occlusion of peripheral arteries -> High Coronary Risk Mohical Conditions > - ↑ TG ↳ Cirhosis , diabetes, genetic factors , hyperlipidemia hypothyroidism, , ↓ especially if not controlled Nephrotic Syndrome disease Pancreatitis R Kilmy , > due ↓ TG - to Low fet Diet , : Hyperthyroidism , Malabsorption Syndrome, Malnutritution Carbohydrates > ↑TGm usually > occur with ↓ Cholesterol TREATMENT f I Medications Lifestyle > Cholesterd Lipid profile Tests - > - HDL - LDL ↳ TG. 1 Determination ofSerum Cholesterd Total Cholesterol (TC) > All - Cholesterd in Various Lipoproteins 25 % (Minor fraction) >. - As free Cholesterd - > - Most occurs as "fatty acyl ester" esterified Cholesterd - > principle Red Color Complex ↓ Quinonimine 2. Determination ofSerum Triglycerides ↳ Collecting Blood for Lipoproteins , TG => & After 12 hrs fasting ↳> No medications/No stres ↳ Within 6 Weeks -> after major illness > Acute MI - 0 D Test Serum TG , mg/d) =. x100 OD. Sta & Determination of. Serum HDL The apoB containing lipoproteins in the specimen are reacted with a blocking reagent that renders them non-reactive with the enzymatic cholesterol reagent under conditions of the assay. The apoB containing lipoproteins are thus effectively excluded from the assay and only HDL-chol is detected under the assay conditions. It is a precipitation assay that involves precipitation of non-HDL lipoproteins in the presence of phosphotungstic acid-MgC12. After centrifugation, HDL cholesterol content of the supernatants will be measured enzymatically according to the method described above (cholesterol oxidase peroxidase) or Enzyme Colorimetric Method. > Principle - After precipitation with a polyanionic compound, In the presence of water, cholesteryl ester is hydrolyzed by cholesteryl esterase to give cholesterol and fatty acid. The cholesterol formed is further oxidized into 4-cholesten-3-one and H202 by cholesterol oxidase. In the presence of peroxidase, H202 is broken down to release 02 which in the presence of phenol, combines with 4-aminoantipyrine to form a red quinine color complex which is read spectrophotometrically at 500m. CALCULATIONS ↑ ↳ LDL = Total Cholesterol - HDL-)I) FireGewald Equation ↳ ULDL