Pre-lab Reading 2-Enzymatic Activity Using Baker's Yeast-1.pdf

Far Eastern University | Institute of Health Sciences and Nursing Biochemistry for Health Allied Sciences Laboratory Pre-lab Readings 2024 Edition Factors Affecting the Enzyme Activity Factor Descriptio...

Far Eastern University | Institute of Health Sciences and Nursing Biochemistry for Health Allied Sciences Laboratory Pre-lab Readings 2024 Edition Factors Affecting the Enzyme Activity Factor Description Example Temperature Enzymes have an optimal Optimal Temperature: Human enzymes typically work best at temperature range. Too high or too around 37°C. At higher temperatures, enzymes like amylase can low temperatures can reduce denature. activity. Low-low activity due to reduced kinetic energy Optimal- Maximum Activity due to optimal kinetic and enzyme structure. High-Reduced activity or denaturation to enzyme structure disruption pH Each enzyme has an optimal pH Optimal pH: Pepsin in the stomach functions best at a pH of 1.5 to range. Deviations can alter enzyme 2.5, whereas trypsin in the small intestine works best at a pH of 7.5 structure and function. to 8.5. Low (acidic)-reduced activity if outside optimal pH range Optimal- Maximum Activity at enzyme’s specific pH High (basic)-Reduced activity if outside optimal pH range Substrate Increasing substrate concentration The reaction rate of catalase increases with higher concentrations Concentration typically increases the rate of of hydrogen peroxide until all enzyme active sites are occupied. reaction until the enzyme becomes saturated. Low-low activity due to fewer substrate molecules Optimal- Increased activity as more substrate molecules bind High-Maximum activity as enzyme sites are saturated disruption  Far Eastern University | Institute of Health Sciences and Nursing Biochemistry for Health Allied Sciences Laboratory Pre-lab Readings 2024 Edition Enzyme More enzyme molecules generally In a reaction with fixed substrate concentration, increasing enzyme Concentration lead to a higher rate of reaction, concentration will accelerate the breakdown of glucose by assuming substrate is available. invertase. Low-low activity due to fewer enzyme molecules Optimal- Increased activity with more enzyme molecules High-High activity assuming sufficient substrate is present