Practical Oral Biology HPT 231 PDF

‫بسم هللا الرحمن الرحيم‬ Practical Oral Biology HPT 231 Tooth Development  4 1 2 3 1-upper tooth germ 2- lower tooth germ Coronal section in 3- t...

‫بسم هللا الرحمن الرحيم‬ Practical Oral Biology HPT 231 Tooth Development  4 1 2 3 1-upper tooth germ 2- lower tooth germ Coronal section in 3- tongue face 4- palate  Dental Lamina Oral epithelium Dental lamina Palate Tongue Ectomesenchymal tissue Dental lamina  Dental lamina Oral epithelium Ectomesenchymal tissue Bud Stage Oral epithelium Dental lamina Enamel Organ Dental papilla Dental sac Tooth germ Bud Stage Oral epithelium Dental lamina Enamel organ Dental sac Dental papilla Oral epithelium Bud stage Dental lamina Enamel organ Dental sac Dental papilla ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl Oral epithelium Cap Stage Dental lamina Outer enamel epithelium Stallate reticulum Inner enamel epithelium Cell free zone Dental papilla Dental sac Cap Stage 1 2 8 3 4 7 5 6 1- Oral epithelium 5- Inner enamel epithelium 2- Dental lamina 6- Cell free zone 3- Outer enamel epithelium 7- Dental papilla ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl 4- Stellate reticulum 8- Dental sac  Cap Stage 1 2 3 4 9 6 5 8 5- Inner enamel epithelium 1- Oral epithelium 6- Cell free zone 2- Dental lamina 7 7- Dental papilla 3- Outer enamel epithelium 8- Dental sac ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl 4- Stellate reticulum Cap Stage ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl Cap Stage Enamel cord Enamel knot ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl Oral epithelium Cap Stage Outer dental epithelium Dental lamina Enamel knot Stellate reticulum Cell free zone Inner dental Epithelium Dental papilla Dental sac  8 Early Bell Stage Dental sac Outer Enamel epithelium 1 Stallate reticulum 2 Stratum Intermedium 3 Inner Emanel epithelium 4 7 Dental papilla Cell free zone 5 Cervical loop 6 10 Early bell stage 9 1 4 8 3 5 2 7 6 1- Outer enamel epithelium 7- Dental papilla 2- Stellate reticulum 8- Dental sac 3- Stratum intermedium 9- Lateral Dental lamina 4- Inner enamel epithelium 10- Oral epithelium 5- Cell free zone 6- Cervical loop  12 Early bell stage 1 9 10 3 2 4 11 6 5 8 1- Outer enamel epithelium 7 7- Dental papilla 2- Stellate reticulum 8- Dental sac 3- Stratum intermedium 9- main dental lamina 4- Inner enamel epithelium 10- lateral dental lamina 5- Cell free zone 11- successional dental lamina 6- Cervical loop 12- Oral epithelium Early bell stage 7 6 8 5 1 4 2 3  Early bell stage 4 2 1 6 3 5 1- Outer enamel epithelium 2- Stellate reticulum 3- Stratum intermedium 4- Inner enamel epithelium 5- Cell free zone 6- Dental papilla  Early bell 12 stage 9 1 2 1- Outer enamel epithelium 2- Stellate reticulum 3 10 3- Stratum 4 intermedium 4- Inner enamel 13 epithelium 11 5- Cell free zone 6- Cervical loop 7- Dental papilla 8- Dental sac 5 9- main dental lamina 10- lateral dental 6 lamina 11- successional dental 7 lamina 8 12- Oral epithelium 13- Odontoblasts ate Bell Stage 5 2 1 4 3 6 7 8 9 10 12 13 11 1- outer enamel epithelium, 2- stellate reticulum, 3- stratum intermedium, 4- ameloblast, 5- enamel matrix, 6- dentin, 7- predentin, 8- odontoblast, 9- cell free zone, 10- inner enamel epithelium, 11- cervical loop, 12- dental papilla, 13- dental sac. ate Bell Stage 10 1 4 2 5 3 6 7 8 9 1- outer enamel epithelium, 2- stellate reticulum, 3- stratum intermedium, 4- ameloblast, 5- enamel matrix, 6- dentin, 7- predentin, 8- odontoblast, 9- dental papilla, 10- dental sac.  Late Bell Stage Outer dental epithelium Stallate reticulum Dental papilla ate Bell Stage 6 5 7 9 8 2 1 10 3 4 1- outer enamel epithelium, 2- stellate reticulum, 3- dental sac, 4- cervical loop, 5- enamel matrix, 6- enamel space, 7- dentin, 8- predentin, 9- odontoblast, 10- dental papilla ate Bell Stage 1 3 4 2 6 5 8 7  Late Bell Stage Ameloblasts Ameloblast Enamel matrix Dentin Predentin Odontoblasts Odontoblasts Dentin Dental Papilla  Late Bell Stage ☻Hard tissue formation ( Dentin and Enamel). Ameloblast ate Bell Stage ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl ate Bell Stage ate Bell Stage 3 1 2 4 9 5 6 7 8 1- Reduced Enamel epithelium, 2- Enamel space, 3- Dentin, 4- predentin, 5- Enamel matrix, 6- Odontoblast, 7- Dental papilla (pulp), 8- Dental sac, 9- permanent tooth germ ROOT FORMATION 1 2 3 4 5 9 6 7 8 1- Reduced Enamel epithelium, 2- Enamel space, 3- Dentin, 4- predentin, 5- Odontoblast, 6- Dental papilla (pulp), 7- Dental sac, 8- Epithelial Diaphragm, 9- Epithelial root sheath of hertwig  ROOT FORMATION 3 2 4 1 5 6 7 1- Dental papilla (pulp), 2- Odontoblast, 3-Dentin, 4- Enamel space, 5- Reduced Enamel epithelium, 6- Epithelial Diaphragm, 7- Dental sac  ROOT FORMATIO 1 5 2 3 4 6 1- Odontoblast, 2- Predentin, 3-Dentin, 4- Epithelial Diaphragm, 5- Dental papilla (pulp), 6- Dent  ROOT FORMATION 1 2 6 3 4 5 7 1- Odontoblast, 2- Predentin, 3-Dentin, 4- Epithelial root sheath of hertwig, 5- Epithelial Diaphragm, 6- Dental papilla (pulp), 7- Dental sac  6 ROOT FORMATION 1 7 5 2 4 8 3 8 1- Odontoblast, 2- Predentin, 3-Dentin, 4- Dental papilla (pulp), 5- Cementum, 6- Cementoblast, ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl 7- Dental sac, 8- Epithelial rests of Malassez Enamel  L.S. Ground Section in Crown E D Incremental lines of Retzius  L.S. Ground Section in Crown E D E D Dentino-enamel junction T.S. Ground L.S. Ground Section in Crown Section in Crown Enamel lamella L.S. Ground Section in Crown E D T.S. Ground Section in Crown Enamel tuft T.S. Ground Section in Crown Enamel spindle T.S. Ground Section in Crown E D T.S. Ground Section in Crown  Enamel Lamella Enamel Tuft Enamel Spindle E DEJ T.S. Ground Section in D Crown Hunter Schreger Bands Rodless enamel Hunter Schreger Bands Gnarled Diazone Enamel Rodless enamel Parazone Rodless enamel DEJ Hunter Schreger Bands A- parazone B- Diazone B C- DEJ C T.S. Ground Section in Crown 1- DEJ 2-incremental lines of retzius 3- enamel lamella 4- enamel tuft 5- dentinal tubules Dentin  Some Types of Dentin Mantle dentin Tertiary dentin Primary dentin Secondary dentin Predentin ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl Oral Histology, 5th edition, A R Ten Cate Ground Sections L.S. Ground Section in Crown Dentinal tubules E Longitudinal Ground E section in a tooth Red: DEJ D Orange: dentinal Tubules (DT) Blue: Tomes granular layer Purple: Cementoenamel junction Pink: Pulp Space C  E A: 2ry curvature of Dentinal tubules B: DEJ C: Mantel Dentin D: DT of circumpulpal Dentin E: Enamel  1 E 4 3 2 1- DEJ 2- Dentinal Tubules TRANSMISSION 3- Odontoblastic process ELECTRON MICROSCOPE 4- Terminal branching OF DENTIN  1 3 2 1- Dentinal Tubules 2- Periodontoblastic space SCANNING ELECTRON 3- Peritubular dentin MICROSCOPE OF DENTIN  A: Peritubular dentin SCANNING ELECTRON B: Intertubular dentin MICROSCOPE OF DENTIN C: Periodontoblastic space  SCANNING ELECTRON MICROSCOPE OF DENTIN 1 2 3 4 1: Peritubular dentin 2: Intertubular dentin 3: Periodontoblastic space 4: Odontoblastic Process L.S. Ground Section in Crown A: Dentin B: Enamel C: Dentinal Tubules D: DEJ E: Enamel spindle  L.S. Ground Section in Crown F E A: Incremental lines of Retzius B: DEJ C: Dentinal Tubules D: Dentine E: Enamel F: Enamel Lamella  T.S. Ground Section in Crown E 1 2 3 4 Interglobular Dentin Spa 1- Enamel tuft 2- DEJ 3- Dentinal tubules of Mantel Dentin 4- dentinal tubule of circumpulpal dentin  1- Dentinal tubules of Mantel Dentin 2- Dentinal tubule of circumpulpal dentin 1 3- DEJ 4- Interglobular Dentin 3 Space 5- Circumpulpal Dentin L.S. Ground Section in Crown 2 4 5 L.S. Ground Section in Crown 1 2 1- DEJ 2- Enamel 3- cementoenamel junction 4- dentinal tubules (dentin) 5- Cementum (acellular) 4 Tome’s 3 granular Dentin 5 ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl L.S. Ground Section in Crown (Dentinal tubules) Tomes Granular Layer Dentinal tubules of mantel dentin Dentinal tubules of circumpulpal dentin Enamel Lamella S p space a c e Incremental lines of Retzius L.S. Ground Section in Crown L.S. Ground Section in Crown DEJ Enamel Interglobular dentin space Incremental lines of Retzius Dentinal tubules of mantel dentin granular layer Tome’s L.S. Ground T.S. Ground Section Section in in Crown Enamel Crown lamella Dead Tracts & DEJ Sclerotic Dentin  L.S. Ground Section in Crown 5 1 3 2 4 1- dead tract , 2- mantel dentin, 3- dentino-enamel junction, 4- dentinal tubules of circumpulpal dentin, 5- incremental lines of Retzius  E D B A F C G A- dead tract, B- reparative dentin, C- sclerotic dentin, D- DEJ, E- Enamel, F- pulp space, G- Acellular cementum L.S. Ground Section in Crown Decalcified Section in A Tooth Dentin ,Predentin &Odontoblasts Line of demarcation P.D. S.D. Dentinal tubules Regular secondary dentin Dentinal tubules  Pulp core Pulp core Reparative Dentin 1ry dentin circum pulpal Line of demarcation Predentin Pulp  Decalcified section In A Tooth alcified Section in A Tooth Pulp in situ Dentin PreDentin Pulp in situ Odontoblast 5 4 3 6 4 5 2 1 1 2 3 1- dentin (dentinal tubules), 2- Predentin, 3- Odontoblast, 4- Cell free zone, 5- Cell rich zone, 6- Pulp core  Cell free zone Dentin (Dentinal tubules) Pulp core Cell rich zone Predentin Odontoblast  1- Dentin True pulp stone 1 2- Predentin 3- Odontoblast 2 4- Pulp core 5- Cell free zone 4 6- Cell rich zone 3 4 False pulp stone 2 3 5 1 6  1 ffuse Calcifications 2 1 2 1 True pulp stone 2 3 4 Cementum Ground Section  L.S. Ground Section in Root Acellular Tome’s granular cementum layer Dentinal tubules Dentin  L.S. Ground Section in Root Sharpey’s fiber space Tome’s granular layer Incremental lines of salter Dentinal tubules  L.S. Ground Section in Root Tome’s Cellular Cementum granular layer Dentinal tubules Dentin Lacuna of cementocyte  L.S. Ground Section in Root Lacuna of cementocyte Incremental lines of salter Cellular Cementum  L.S. Ground Section in Root Acellular cementum Cellular cementum Lacuna of cementocytes Granular layer of Tomes Dentinal tubules ©Copyright 2007, Thomas G. Hollinger, Gainesville, Fl  Tome’s granular layer Dentinal tubules ? ? ? ? Lacuna of cementocyte ? L.S. Ground Section in Tooth  L.S. Ground Section in Root ENAMEL DENTIN (dentinal tubules) Afibrillar cementum Acellular cementum Tome’s granular layer Decalcified Section Lacuna of cementocytes Dentin Predentin Pulp  Lacuna of Dentino-cemntal junction cementocytes PDL Incremental lines Cellular of salter cementum Periodontal ligament  Decalcified Section Gingival Alveolar crest Horizontal Interradicular Oblique Oblique Apical Decalcified Section Transeptal Fibers Acellular Dentin cementum Alveolar Interstitial crest tissue Horizontal  Decalcified Section Interstitial tissue Alveolar Crest Acellular cementum Dentin & Horizontal Fibers  Decalcified Section Crest of alveolar bone Interstitial tissue oblique fibers  Decalcified Section Oblique Fibers  Decalcified Section Apical fibers  Decalcified Section Acellular cementum Interstitial tissue  Decalcified Section Dentin Inter-radicular fibers Acellular cementum Interstitial tissue  Incremental lines of salter Cementicle Acellular cementum Decalcified Section  Decalcified Section Epithelial rests of Malassez ?? Acellular cementum Dentin  Decalcified Section Alveolar Crest  a b C B A B A  C B A  A H C B D F E G