Practical Bio 1 PDF

Biochemistry Laboratory safety PRACTICAL (1) DR. El-Sawy 0  Biochemistry Laboratory safe...

Biochemistry Laboratory safety PRACTICAL (1) DR. El-Sawy 0  Biochemistry Laboratory safety Hazards in the laboratory :  Exposure to blood, body fluids & specimens contains Biological pathogenic bacteria & viruses. Chemical  Acids, alkalis and toxic chemicals. Radiological  Ineffective radioactive waste disposal.  Fire. Accidents  Electrical. General Safety Rules : 1. Read instructions carefully before starting your experiment. 2. Wear safety goggles to protect your eyes from chemicals, heated materials, or things that might be able to shatter. 3. Wear a protecting lab coat, hand gloves, face mask and hair cap. 4. After handling chemicals, always wash your hands with soap and water. 5. During lab work, keep your hands away from your face. 6. Never mix chemicals together unless you are told to do so. 7. Notify your teacher if any spills or accidents occur. 8. Clean up your lab area at the end of your experiment. DR. El-Sawy 1  Biochemistry Laboratory safety Glassware Safety : 1. Broken glassware should be disposed of in a special glass disposal container. 2. When pouring liquids into glassware, make sure it is resting on a table at least a hands breadth from the edge. 3. If a piece of glassware gets broken, notify the teacher. Heating Safety : 1. Use protective gloves to handle hot objects. 2. Never reach across an open flame. 3. Always point top ends of test tubes that being heated away from people. 4. When heating a test tube, move it around slowly over the flame to distribute the heat evenly. 5. Only glassware that is thoroughly dry should be heated. 6. Heat glassware by placing it on a wire gauze platform on a ring stand. Do not hold it in your hand. First Aid : Burns  Immediately flush with cold water.  Do not touch an open wound without safety gloves.  Pressing directly on minor cuts will stop bleeding in Cuts & bruises a few minutes.  Apply cold compress to bruises to reduce swelling.  Flush eyes immediately with plenty of water for several minutes. eyes  If a foreign object is lodged in the eye, do not allow the eye to be rubbed. DR. El-Sawy 2