Hematology Practical Lab 2 PDF

Hematocrit test Asst. prof. Dr Ramiar kamal kheder/Mr. Ahmed Bahram Wlia Hematology practical Fall semester week 2 Date/ 7-oct-2024 Objectives  Introduction of Hematocrit test  Principles and methods currently use...

Hematocrit test Asst. prof. Dr Ramiar kamal kheder/Mr. Ahmed Bahram Wlia Hematology practical Fall semester week 2 Date/ 7-oct-2024 Objectives  Introduction of Hematocrit test  Principles and methods currently used for direct measurement of PCV  Specimen collection  Materials requirement for PCV test  Procedure  Clinical problem  Source of error Hematocrit (Hct) or Packed Cell Volume (PCV) or Erythrocyte Volume Fraction (EVF)  When heparinized blood (heparin is an anticoagulant) is centrifuged, the red blood cells become packed at the bottom of the tube, while the plasma is left at the top as a clear liquid. The ratio of the volume of packed red cells to the total blood volume is called the hematocrit.  HCT measures the volume of packed red blood cells (RBC) relative to whole blood, also known as packed cell volume (PCV).  Hematocrit is the proportion of pure RBCs in whole blood.  It's a simple test that identifies conditions Principle: When anticoagulated blood is centrifuged in a hematocrit tube at high speed, the RBC which are heavier than WBC, Platelet & plasma sediments at the bottom. The red cell column is called PCV or hematocrit. PCV test can be done by two methods: 1. Macro hematocrit: Wintrobe hematocrit 2. Micro hematocrit: Heparinized capillary is used. In the case of difficulty drawing sufficient blood, this method is used. Sample collection Capillary blood Venipuncture Heel puncture Capillary blood is best A healthcare professional In the case of infants, taken from the radial collects a blood sample the blood can be taken side of the middle using a needle. The from the infant’s heel. fingertip sample is then centrifuged to separate the components. Reading PCV Value The capillary tube should be parallel to graduation and lower level of RBCs on zero line of the scale and the upper level of the scale and the upper level of the clear plasma on 100 % line). Do not include the buffy coat (WBCs and platelets) when reading PCV value. Thank You