Plant Biotechnology Mid-Term Exam Papers 2023-2024 PDF

# M.Sc. Plant Biotechnology (First Semester) Mid-Term Examination, 2023-24 ## PBT-502: Principles of Biotechnology - Time: 2 hrs - Credits: 3 (2+1) - Max. Marks: 30 **Note:** Attempt any five questions. Each question carries equal marks. 1. Give various strategies to make a plant herbicide toler...

# M.Sc. Plant Biotechnology (First Semester) Mid-Term Examination, 2023-24 ## PBT-502: Principles of Biotechnology - Time: 2 hrs - Credits: 3 (2+1) - Max. Marks: 30 **Note:** Attempt any five questions. Each question carries equal marks. 1. Give various strategies to make a plant herbicide tolerance. Discuss about the mechanism of glyphosate tolerant plant with the vector used for transfection. 2. Enlist various direct transformation methods. Explain in detail about calcium phosphate and pollen mediated gene transfer method. 3. Define plasmid and give its different types. Illustrate various artificial chromosomes as a vector with figure. 4. Describe cryopreservation. Explain in details about the techniques used for cryopreservation. 5. What is star activity? Describe different types of restriction enzymes. Differentiate between Isoschizomers and Neoschizomers. 6. Write short notes on any two of the followings - Applications of germplasm conservation - Particle gun bombardment - pBR 322 ## PBT-507: Transgenic Plant Production Technology - Time: 2 hrs - Credits: 3 (2+1) - Max. Marks: 30 **Note:** Attempt any five questions. Each question carries equal marks. 1. Enlist various properties of RNA to qualify as a enzyme. Give schematic representation of ribozyme approach to prevent the expression of gene. 2. Design the gene construct and explain the mechanism of *Agrobacterium*-plant cell interaction with diagramme. 3. Enlist various transgene showing resistance to specific insects and give the reasons for low level expression of *cry* genes in plant. 4. Illustrate the T-DNA and *vir* region of Ti plasmid with figure. 5. Define promoter with its types. Explain in detail about *Agrobacterium* mediated gene transfer method. 6. Write short notes on any two of the followings. - Reporter genes - *Cry* proteins and its mode of action - Selectable marker ## PBT-506: Plant and Microbial Diversity - Time: 2 Hours - Credits: 3 (2+1) - Max. Marks: 30 **Note:** Attempt any six (All Questions carry equal marks). 1. Discuss the important role of CIMMYT and NBPGR research institutes in response to research activity and food security. 2. Describe the genetic diversity and its importance in crop plants with suitable example. 3. Explain various *in-situ* approaches which are being used to conserve the crop plants along with its merits and demerits in brief. 4. Differentiate the land races with modern cultivars with its merits and demerits in brief. 5. Explain the important features of germplasm, major types and its importance in crop improvement programme. 6. Give your comments on germplasm activities performed by research agencies in reference to crop improvement and food security. 7. Write a short note on any two the following. - Primary and secondary gene pool - Wheat crop evolution. - Golden Rice - Basic and derived genetic stocks ## PBT-511: Molecular Approaches to Plant Physiology - Time: 2 hrs - Credits: 3 (2+1) - Max. Marks: 30 **Note:** Attempt any five questions which carry equal marks 1. Briefly explain the phytoremediation, mechanism and its different methods with suitable example. Also write the importance of phytoremediation. 2. Describe about energy conserving phase of glycolysis along with its schematic diagram. 3. Discuss the Citric Acid Cycle by describing the number of CO2, NADH/FADH and ATP released during oxidation of one molecule of pyruvate in mitochondria. 4. Diagrammatically describe the of pentose phosphate pathways in brief and also discuss about the hexose monophosphate shunt and its role in plant metabolism. **OR** Describe the Seed Germination, Seed Viability and Apoplast and Symplast in brief. 5. Explain the mechanism of absorption/uptake of minerals and water by plants and its significance in brief. 6. Describe any two of the following - Succinate dehydrogenase (Complex II) - Cytochrome bc1 complex (Complex III) - Seed dormancy 7. Answer any two of the following in respect of classical biochemical effect associated with aerobic glycolysis- - Pasteur Effect - Crabtree effect - Warburg Effect