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Microscopy Teaching Presentation PDF

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Summary

A presentation on various types of microscopes, including their functions, parts, and applications. It is a helpful tool for understanding the principles and uses of different microscope types. Includes visuals and descriptions.

Full Transcript

http://www.udel.edu/biology/ketcham/microscope/ scope.html Compound Light Microscope Dissecting Microscope Transmission Electron Microscope (TEM) Scanning Electron Microscope (SEM) http://www.mos.org/sln/sem/seminfo.html On/ off arm illuminator base...

http://www.udel.edu/biology/ketcham/microscope/ scope.html Compound Light Microscope Dissecting Microscope Transmission Electron Microscope (TEM) Scanning Electron Microscope (SEM) http://www.mos.org/sln/sem/seminfo.html On/ off arm illuminator base rheostat Ocular 10X nosepiece objectives Interpupillary distance Mechanical stage → Stage → Course adj. knob Fine adj. knob Condenser lens Filter holder Control for Control knobs for condenser stage Lever for Iris Diaphragm -> ← Iris Diaphragm scanning 4X High power medium 40X power 10X Oil immersion 100X magnification / numerical aperture (NA) mechanical tube length / coverslip thickness Field of View Parfocal –remains in focus when objectives change Parcentral – center of field of view remains same Working distance 40 x 100 x 400 x Virtual microscope www.udel.edu/biology/ketcham/ microscope/scope.html

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