Microbiology 2nd Internal PDF

## Multiple Choice Questions - (10 X 0.5 = 5.0) a. The technique of deliberately infecting the young children by rubbing the small pox scab on the skin, practiced by ancient Chinese's was known as... - **Immunization** b. Long hinge region is Characteristic of: - **IgG** c. Unicellular Fu...

## Multiple Choice Questions - (10 X 0.5 = 5.0) a. The technique of deliberately infecting the young children by rubbing the small pox scab on the skin, practiced by ancient Chinese's was known as... - **Immunization** b. Long hinge region is Characteristic of: - **IgG** c. Unicellular Fungi are known as: - **Yeast** d. Antibodies are produced by: - **Immunoglobulin X cells** e. PAGE is an acronym for: - **Polyacrylamide gel electrophoresis** f. The process of promoting phagocytosis is called: - **Opsonisation** g. Any substance that can induce an immune response in humans or animals is called: - **Antigen** h. Antibodies are produced during Primary Immune response. i. EcoR1, BamHI and HindIII are the class of enzymes known as: - **Restriction endonucleases (Type II)** j. The part of an antigen that is directly involved in receptor-antigen interaction is called: - **Epitope** ## Encircle the correct answer - (10 X 0.5 = 5.0) ### Complement used for CFT is derive from a) Mice b) **Rabbit** c) Guinea pig d) Hamster ### Complementary determining regions are present on: a) **Antigen** b) Constant region of antibody c) Hypervariable region of antibody d) All of above ### Yeast conversion form of Dimorphic fungi is possible with all except: a) H.capsulatum b) B.dermatitides c) **S.schenckii** d) Aspergillus ### Serum sickness is an example of: a) Type I b) **Type II** c) Type III d) Type IV ### The method which utilizes the altered dNTPs in addition to the normal ones for determining the sequence of a segment of DNA: a) Sanger sequencing b) **Maxam Gilbert sequencing** c) Next generation sequencing d) DNA Microarray ### Then tissue is transplanted between genetically different members of the same species, it termed as: a) **Allograft** b) Xenograft ## Fill in the Blanks - (10 X 1.0 = 10.0) ### Scientist was awarded noble price for the discovery of PCR: - **Kary Mullis** ### Pepsin digestion of immunoglobulin result in generation of: - **Fab** ### FAT is an acronym for: - **Fluoroscent Antibody Technique** ### During PCR, Taq polymerase adds new nucleotides at which end of the primer: - **3'-OH** ## Match the following: - (10 X 1.0 = 10.0) | | | | ------------- | ------------- | | a) Polymerase Chain Reaction | Kary Mullis | | b) Cytotoxic T-cells | CD8 | | c) Agglutination x | Particulate antigene | | d) Southern Blotting | DNA | | e) Helper T-cells | CD4 | | f) Phagocytosis | Metchnikoff | | g) Candida | Germ tube | | h) Precipitation | Soluble antigen | | i) Northern Blotting | RNA | | j) C. neoformans | Pigeon droppings | ## Write briefly about the following (Any FIVE) - (10 X 1 = 10) - Different types of Vaccines - RNA - Soluble antigen - Germ tube - Metchnikoff - CD4 - DNA - Particulate antigene - CD8 ## Differentiate between Innate and Adaptive Immunity - (5 X 2.0 = 10) ## Principle of ELISA ## Western Blotting ## Basic steps of Gene cloning (Flow chart) ## Dimorphic Fungi ## Dermatophytes ## Long Type (Attempt any TWO) - (2 X 5.0 = 10) a) Discuss in detail about the structure of IgG with a neat diagram. b) Define Mycotoxicosis and discuss in detail about different mycotoxins. c) Explain in detail the Principle of Polymerase chain reaction? What are the various components of a PCR reaction mixture? Explain the significance of each component?