Lecture_2_protein electrophoresis.pdf

Protein Electrophoresis Lecture 2  Plasma Protein (Electrofocusing) technique for separating different molecules by their electric charge differences. It is a type of zone electrophoresis Alpha-1-antitrypsin Alpha-1- fetoprotei...

Protein Electrophoresis Lecture 2  Plasma Protein (Electrofocusing) technique for separating different molecules by their electric charge differences. It is a type of zone electrophoresis Alpha-1-antitrypsin Alpha-1- fetoprotein Haptoglobin Ceruloplasmin (Transferin) (Lipoproteins)  Alpha-1-Globulins Alpha-1-antitrypsin Alpha-1-fetoprotein liver cancer marker birth defects of the brain Down’s and spinal cord Acute phase Emphysema Neural tube Syndrom Pregnancy In Neonates Defects (Spina extra copy of type of chronic Bifida) in Amniotic chromosome 21 (Done on obstructive Fluid + Maternal Serum maternal serum) pulmonary disease  Alpha-2-Globulins Haptoglobin Ceruloplasmin (binds free hemoglobin) (Transports _____) a. Wilson’s a. Acute phase Hemolysis a. Acute phase b. Liver b. Nephrotic b. Pregnancy disease Disease rare inherited disorder Copper builds Syndrome c. Transfusion reactions up in the liver and injures liver tissue.  Normal Protein Electrophoresis Patterns How does the abnormal Protein Electrophoresis Patterns Look like?  Some Common abnormal Protein Electrophoresis Patterns Acute Inflammation Monoclonal Gammopathy Found in association with multiple myeloma. They also occur in other benign and malignant conditions. Active Cirrhosis  Nephrotic Syndrome Hypogammaglobulinemia lack of B-lymphocytes and a resulting low level of immunglobulins (antibodies) in the blood. 1. What is multiple myeloma and how is it diagnosed? 2. What are protein S and C 3. Differences between nephrotic and nephritic syndrome