Lecture 11: Regulation of Gene Expression PDF

 immunoprecipitated with an anti to a specific modification isolated DNA sequences analyze whiteheistsput lysine H acetyl reduces...

 immunoprecipitated with an anti to a specific modification isolated DNA sequences analyze whiteheistsput lysine H acetyl reduces DNA se 1 sensitii transcriptionally inactive sodiumbutyrate cellular deacetylase activits activate theexpression of previously silenced cellulargene opening  histone acetyltransferas CHAT or KATSA CREEP CBP transcriptional co activator p 300 coactivator TAF250 subunit of the TFIID transcription factor basal HAT ATF2 nuclear receptor Co repressor thyroid hormone receptor absent Sin HDAC  MeCPa CG dinucleotides repressive Mesoderm cell Myoblast Myocytes Myotubes MyFibers StemCells themselves be regulated myth 2 maturemyotubes phosphorylate dytoplasm  theassociati ofthehistones witheach other tails looser improved access toDNA displacement of histones activators other regulatory molecules delffittion inhibited  Brg 1 bromodomain lysine open  lysine and arginine ve more complex  HU R 3 nuclear hormone recently 113 ecdosyre dependent K4 Bative 43 k 27 113 9 27  polycomb comple H3 9 and 27 trithorax H3 4 9 and 27 HPI Heterochromatic Protein1 H3 on tightly page chromatin Histonemethyltransferase enzyme K9 methylation of 13 spread insulator propagated through cell division randomly divided unmethylated histone H3 HPI histonemethyltransferase methylated  PC 1222 Polycomb Repressive complexes PRC2 polycomb complex histone H3 trimethylated on 127 activates histone methyltransferase within PRC complex histone H3 on 27 propagation paradisibitesSister p thinchip gatingandtomedasf.tnchitlins stably inherited allow the transmission of DNA methylation patterns altered by enzymes or by Itg methe inhibiting me of new nucleosomes Chromodomain tight packing open  NURF nucleosome remodellingfactor PHD methylation K4 13 open H2A and H2B 5 10 to repression polycomb protein complex  opposite me of histone H3 on KU and 79 Ku and 79 momethylated trimethylated  opposing oppose inhibits one Profits initial RNA trans failitate chromatin transcription blocks promotes transcriptional repression dearetylate histones closed Serive or theonine open heat shock H3  H3 Serve to C Fos andEmge  90rigjfiasefam.is p coffin lowingsyndrome developmental defect  H1 reduces HPI  O linked n acetylglucosamine CO G Nac Serine 43 112 1B  Serine112 of histone 1213 HB 120 in IB Hz on KU and79 demethylation of methylcytosine in DNAbyTET 5 hydroxymethylcytosine Signel For recruitment open Citrullination arginine compaction H p Ñ G I one