Lecture 10.5 Translation II 2021 PDF
Document Details
Uploaded by SmittenRabbit3769
Johns Hopkins University
2021
Tags
Summary
This document appears to be a lecture on translation, likely from a university or college course. It contains information on the topic of translation regulation and discusses ribosome profiling techniques. The document also includes topics on initiation and elongation regulation within the process of protein synthesis.
Full Transcript
**[Translation II ]** **Regulation:** Changes in thermodynamics and kinetics of the core steps. **Understanding translation:** Polysome profiling (know how to draw this!) Y axis is [A260] absorbance, X axis is sedimentation in sucrose gradient. There's a big hump of RNP/small nucleic acid (junk),...
**[Translation II ]** **Regulation:** Changes in thermodynamics and kinetics of the core steps. **Understanding translation:** Polysome profiling (know how to draw this!) Y axis is [A260] absorbance, X axis is sedimentation in sucrose gradient. There's a big hump of RNP/small nucleic acid (junk), then a small subunit (30s) hump, large subunit (50s) hump, monosome (70s) hump, and polysomes. Process 1. 2. 3. 4. Informative of: - - **Ribosome profiling** Process 1. 2. 3. - - - **Initiation regulation** Initiation is the rate-limiting step: mRNAs have fewer ribosomes than they could potentially hold. 1. - - - - - - - - - - - - - 2. - - 3. - - - - - **Elongation regulation** 1. - - - 2. - - - - - 3. a. b. **Ribosome-mediated quality control:** - - - - - - - - -
