Gene Cloning and Recombinant DNA Technology Lecture Notes PDF

Gene Cloning and Recombinant DNA Technology This presentation explores gene cloning, a core technique in molecular biology. We'll cover principles, techniques, applications, and ethical considerations. by Ahmed Mansour Alzohairy ‫ أحمد منصور الزهيرى مدير ومؤسس الم...

Gene Cloning and Recombinant DNA Technology This presentation explores gene cloning, a core technique in molecular biology. We'll cover principles, techniques, applications, and ethical considerations. by Ahmed Mansour Alzohairy ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy ‫جامعة الزقازيق‬ - ‫والجينومية‬ preencoded.png Principles of Gene Cloning 1 2 3 4 Isolation Insertion Transformation Selection Target gene is isolated. Gene is inserted into a Vector is introduced into Recombinant clones are vector. a host. selected. preencoded.png ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy ‫ جامعة الزقازيق‬- ‫والجينومية‬ Techniques for Gene Isolation 1 Restriction Enzymes 2 PCR Amplification 3 cDNA Synthesis These enzymes cut DNA at Polymerase chain reaction Complementary DNA (cDNA) is specific sequences. (PCR) amplifies specific DNA synthesized from mRNA. regions. preencoded.png ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy Vectors for Gene Insertion Plasmids Viral Vectors Artificial Chromosomes Circular DNA molecules that replicate Modified viruses used to deliver Large DNA molecules designed for independently. genetic material. cloning large genes. preencoded.png ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy Transformation of Host Organisms Bacteria Yeast Commonly used for their rapid growth and ease of Eukaryotic host suitable for expressing some complex manipulation. proteins. preencoded.png ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy  Screening for Recombinant Clones Blue-White Screening Antibiotic Resistance Uses a color change to Clones with the vector survive identify clones with inserted on antibiotic-containing DNA. media. ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy preencoded.png ‫ جامعة الزقازيق‬- ‫والجينومية‬ Selection and Identification of Recombinants Selection Initial screening isolates potential recombinants. Confirmation PCR or sequencing confirms the presence of the target gene. Characterization Further analysis examines gene expression and function. preencoded.png ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy  Applications of Gene Cloning Medicine Producing insulin and other therapeutic proteins. Agriculture Developing genetically modified crops. Research Studying gene function and regulation. preencoded.png ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy ‫ جامعة الزقازيق‬- ‫والجينومية‬  Ethical Considerations in Gene Cloning 1 Safety 2 Environmental Impact Potential risks of genetically modified Concerns about the spread organisms. of modified genes. 3 Ethical Concerns Debates around genetic manipulation. ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy preencoded.png ‫ جامعة الزقازيق‬- ‫والجينومية‬ Future Developments in Gene Cloning Gene Editing CRISPR technology for precise gene modification. Synthetic Biology Creating artificial genetic circuits and organisms. Gene Therapy Treating genetic diseases by modifying genes. preencoded.png ‫ أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬/‫د‬.‫ا‬ by Ahmed Mansour Alzohairy ‫ جامعة الزقازيق‬- ‫والجينومية‬  ‫‪preencoded.png‬‬ ‫ا‪.‬د‪ /‬أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬ ‫‪by Ahmed Mansour Alzohairy‬‬ ‫والجينومية ‪ -‬جامعة الزقازيق‬  ‫‪preencoded.png‬‬ ‫ا‪.‬د‪ /‬أحمد منصور الزهيرى مدير ومؤسس المركز المصرى للمعلوماتية الحيوية‬ ‫‪by Ahmed Mansour Alzohairy‬‬ ‫والجينومية ‪ -‬جامعة الزقازيق‬