Lab 1 Biology Laboratory Biosafety PDF
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Uploaded by BeauteousQuatrain3542
University of Baghdad
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Summary
This document provides an overview of laboratory biosafety protocols, focusing on the importance of biosafety levels, personal protective equipment (PPE) and general safety rules in a laboratory setting. It also outlines the various levels of containment and recommended safety measures.
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Biology Laboratory biosafety Lab 1 WHAT IS BIOSAFETY According to the World Health Organisation (WHO), "biosafety is a strategic and integrated approach to analysing and managing relevant risks to human, animal and plant life and health and associated risks for the environment. ...
Biology Laboratory biosafety Lab 1 WHAT IS BIOSAFETY According to the World Health Organisation (WHO), "biosafety is a strategic and integrated approach to analysing and managing relevant risks to human, animal and plant life and health and associated risks for the environment. Laboratory biosafety is the the containment principles, technologies and practices that are implemented to prevent the unintentional exposure to pathogens and toxins, or their accidental release It is the discipline addressing the safe handling and containment of infectious microorganisms and hazardous biological materials. The practice of safe handling of pathogenic micro-organisms and their toxins in the biological laboratory is accomplished through the application of containment principles and the risk assessment. Why we need Biosafety? The ultimate aim is to eliminate or minimise biological contamination (prevent the entry of disease - control the spread of disease ) Protective Equipment personal protective equipment (PPE) Safety equipment Personal Protective Equipment (PPE) Determining the level of personal protective equipment (PPE) required can be tricky. When dealing with biohazards and other hazardous materials, you don’t want to take a chance. So how do you know what you need? Outlines the level of PPE required based on the work being done in the lab. The personal protective equipment PPE which protect the face, respiratory system, hands and body from work-related hazards Essential personal protective equipment (PPE)Every Lab Needs Safety goggles Lab coats Protective gloves Shoe Covers Head Caps Biosafety Levels (BSLs) A biosafety level (BSL), or pathogen/protection level, is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility They are individual safeguards designed to protect laboratory personnel, as well as the surrounding environment and community. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4). BSL4 BSL3 BSL2 BSL1 Basic safety rules 1)silence all cell phones. 2)All persons in laboratories, including students, staff, and visitors, shall wear safety glasses, goggles or face shields at all times 3)Eating, drinking, chewing gum, and applying cosmetics are prohibited 4)Do not store food in the same refrigerators or freezers with chemicals, biohazards, or radioactive materials. 5)Appropriate gloves are essential when working with hazardous substances. 6)Wearing an iPod, Bluetooth, or any other device that interferes with hearing is not allowed. 7)Never pipet anything by mouth. 8)The work area must be kept clean and uncluttered. 9)All chemicals should be labeled and stored properly. 10)Never work alone in the laboratory 11)The hazards of chemicals used should be known (flammability, reactivity, stability, and toxicity). 12) Always pay attention to your surroundings and be aware of what others are doing. 13)Remove contaminated gloves before touching common use devices (door knobs, equipment); discard gloves before leaving the laboratory. 14)Always wash hands and arms with soap and water before leaving the laboratory. 15)No smoking!