Hemostasis Evaluation PDF

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Institute of Health Technology, Dhaka

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hematology clinical pathology blood testing laboratory techniques

Summary

This document provides an overview of specimen collection and evaluation in hemostasis testing, touching on contamination, temperature, hemolysis, and equipment. It also covers different anticoagulants and specimen handling for various tests.

Full Transcript

HEM OSTA SIS E V A LU A TIO N 06 SP EC IM EN C O LLE C TIO N I N H EM OST A SIS T ES T IN G Prim ary c o n ce rn i s t o p re ve n t p re m atu re a c tiv a tio n ( fa ls e ly s h ort e n ed t e sts ) o f t h e clo ttin g p ro ce ss 1C O NTA M IN ATIO N W IT H T IS SU E T H RO M BO PL...

HEM OSTA SIS E V A LU A TIO N 06 SP EC IM EN C O LLE C TIO N I N H EM OST A SIS T ES T IN G Prim ary c o n ce rn i s t o p re ve n t p re m atu re a c tiv a tio n ( fa ls e ly s h ort e n ed t e sts ) o f t h e clo ttin g p ro ce ss 1C O NTA M IN ATIO N W IT H T IS SU E T H RO M BO PLA ST IN _ ___________________ i s r e le ase d f o rm i n ju re d c e lls a n d a c tiv a te s t h e _ ____ ____________________ 2IN APPR O PR IA TE C O NTA IN ER G la ss p ro vid es a n eg ativ e ly c h arg ed s u rfa c e a c tiv a tin g t h e _ __________ _____________ a n d attra c ts _ _______________________ U se p oly sty re n e t u b es o r g la ss t u b es c o ate d w it h s il ic o n e 3IM PR O PER T EM PER A TU RE La b ile f a c to rs a t r o om t e m pera tu re - P re m atu re ly a c tiv a te d a t r e f t e m pera tu re - 4H EM OLY SIS P ro lo n g ed t o urn iq uet a p plic atio n ( < 1 m in ute ) M ois tu re o r c o n ta m in atio n U sin g n eed le s w it h t o o s m all a b ore F ro th in g o f s a m ple d ue t o a ir e n try E xp ellin g o f b lo od f r o m t h e s y rin g e t h ro ug h t h e n eed le E xc e ssiv e a n d v ig oro us m ix in g EQ UIP M EN T1NEED LE S IZ E N eed le s w it h s m all b ore s a re m ore l ik e ly t o c au se h em oly sis 2 0 -g au g e n eed le - m ost c o m mon ly u se d f o r h em osta sis s a m ple c o lle ctio n 19 -g au g e n eed le - f o r c o lle ctio n o f 2 0 m L o f b lo od o r m ore 2 1- g au g e n eed le - n arro w o r s m all v e in s 2EV A C UATED T U BES / S Y R IN G ES S yrin g es o r e va c u ate d t u b es s h ould b e s il ic o n e c o ate d AN TIC O AG ULA N TS1SO DIU M O XALA TE Fo rm s i n so lu b le c o m ple xe s o r p re cip it a te s o n ce r e calc if ie d a n d p ro d uce s a n in te rfe re n ce i n O D t e stin g 2T R IS O DIU M C IT R A TE B in d s C alc iu m Pre se rv e s f a c to r _ ____________ m ost s a tis fa c to ry f o r p la te le t a g gre g atio n s tu d ie s, a n tic o ag ula n t o f c h oic e t o d ay R atio : N in e p art s o f b lo od t o o n e n art o f a n tic o ag ula n t ( 9 :1 ) T h is r a tio i s i d eal f o r p atie n ts w it h n orm al h em ato crit A s p ecim en w it h a h em ato crit o ve r . 5 0 L /L , o r a n i n co m ple te ly f il le d t u b e h as e xc e ss _____________________ w hic h c au se s t h e c o ag ula tio n t e sts t o b e _ _________ _____________ C on ce n tra tio n : C oag ula tio n t e sts a re m ore s e n sit iv e t o e xc e ss c it ra te t h an c alc iu m 3.2 % N a C it ra te ( P re fe rre d ) 3.8 % N a C it ra te - O ve ra n tic o ag ula tio n f o r p atie n ts w it h h ig h h em ato crit 3E D TA U nsu it a b le f o r c o ag ula tio n In hib it s f ib rin og en -th ro m bin r e ac tio n Fa c to r i s u n sta b le V i n i t s p re se n ce 4H EP A RIN A cts w it h a n tit h ro m bin I lI a n d i n hib it s a ll s ta g es o f c o ag ula tio n C an b e u se d f o r p la te le t r e te n tio n t e st S P EC IM EN H AN D LIN G A N D P R O CES SIN G1EFFE C TS O F P H C han g es i n P h c an p ro lo n g c lo ttin g t im es M ed ia te d b y l o ss o f C O 2 Sam ple s s h ould b e u n op en ed i f i m med ia te t e stin g i s n ot p ossib le 2T EM PER A TU RE La b ile : F a c to rs V a n d V III P re m atu re ly a c tiv a te d a t 4 °C : V II a n d X I 3C EN TR IF U G ATIO N 20 00 g f o r 1 0 m in ute s t o o b ta in p la te le t p oor p la sm a Only u p per ¾ o f p la sm a l a ye r s h ould b e u se d f o r t e stin g

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