Microbiology Lab - Laboratory 2: Microbiological Tools PDF

MICROBIOLOGY LAB LABORATORY 2: MICROBIOLOGICAL TOOLS Mainly used for chemical preparation. 3. Microscope MICROBIOLOGICAL TOOLS...

MICROBIOLOGY LAB LABORATORY 2: MICROBIOLOGICAL TOOLS Mainly used for chemical preparation. 3. Microscope MICROBIOLOGICAL TOOLS Ocular lens - 10x magnification The various activities and experiments require Objective Lenses: different equipment, apparatus, glasswares, and ○ Scanning - 4x other tools. As such, familiarization with these ○ Low Power O - 10x different materials and their uses is important to ○ High Power O - 40x the students not only for their safety but also for ○ Oil Immersion - 100x the accuracy of the results of their experiments. Always put the total magnification 1. Autoclave 4. Biosafety Cabinet A sterilizing tool that uses pressure to Is one type of biocontainment create saturated steam. equipment used in a lab to provide This destroys microorganisms that personnel, environmental, and product operate on the premise of moist heat protection. sterilization. Protect against biohazardous or The high pressure within the chamber infectious agents and to help maintain raises the boiling temperature of water quality control of the material being while facilitating quick heat worked with. transmission into the deeper components Has 3 levels: of the apparatus. ○ Level 1 - moderate risk; same 2. Laminar Flow Hood with laminar flow hood. Is an enclosed workstation that is used ○ Level 2 - handling pathogenic to create a contamination-free work biological samples. Has UV and environment through filters. HEPA filter. Protect the working environment from ○ Level 3 - gas-tight; ULPA filter. dust and other airborne contaminants For TB by maintaining a constant, unidirectional flow of HEPA-filtered air over the work area. 3. Fumehood Is a ventilated enclosure used to trap and exhaust vapors, gasses, and nanoparticles. MICROBIOLOGY LAB LABORATORY 2: MICROBIOLOGICAL TOOLS OTHER INFO: Autoclave ○ To sterilize through the use of heat and pressure ○ 121 - 134 C Incubator ○ 37 C ○ To store samples for 24-48 hours ○ Always inverted position of Petri dishes to avoid moisture (condensation) Condensed liquid could drip onto the agar surface Agar ○ 30 - 40 C to solidify ○ 100 C to liquefy Sterilizing Tools (aseptic) ○ 45 degree angle ○ Wait for 15 sec Alcohol ○ 70% alcohol on skin ○ 90 - 95% for glasswares Refrigerator ○ To slow or inhibit the growth of microbes.