Biomaterial Degradation Rate - Experimental 2 PDF

Experimental 2 Degradation rate of biomaterials Purpose: Identify the degradation property of biopolymer materials. Introduction The degradation rate of a material is usually matched with the rate of tissue regeneration. This is to ensure that the scaffold can still provide the necessary support...

Experimental 2 Degradation rate of biomaterials Purpose: Identify the degradation property of biopolymer materials. Introduction The degradation rate of a material is usually matched with the rate of tissue regeneration. This is to ensure that the scaffold can still provide the necessary support for cells to regenerate completely. Biodegradable polymers materials have been degraded in vivo by the action of body fluids. They are used for purposes which the implant is prepared to remain in place for a specific duration, in order to achieve a certain function. Its major requirements are the degradation followed by resorption and biocompatibility. There are several factors that influence the overall rate of degradation, in addition to pH and copolymer composition. In general, polymer degradation is accelerated by greater hydrophilicity in the backbone or end groups, crystallinity, lower average molecular weight, and smaller size of the finished device. Experimental Materials Natural biopolymer Synthetic biopolymer Lysozyme enzyme solution Tissue paper Beaker glass Balance digital Sample dimensions as shown in figure 1 Figure 1 sample of swelling degree. The degradation studied were performed to ASTM standard F1635-04a. The degradation rate of samples of dimensions 2cm×2cm, as shown in figure 1, were determined by weighing the dry samples (W₀). Then, the films were immersed in PBS solution containing lysozyme 0.0001g/L and incubated at 37 °C for 24 h. After one day of incubation, the samples were removed from the degradation media, washed with distilled water and dried at room temperature. The weights of the dried samples were recorded as W₄. Calculated The degradation rate of the sample was calculated as: \text{Weight loss (%) = } \frac{(W_0 - W_1)}{W_0} \times 100 where is the dry weight before degradation and the dry weight after degradation. Discussion 1. How do biopolymers degrade? 2. What is biopolymers give two examples? 3. Draw the relationship between degradation with time of samples?