Positive and Negative Controls (Experiment 1) PDF
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Uploaded by SpeedyBarbizonSchool
Tanta University Faculty of Medicine
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Summary
This document describes experimental procedures for a biology experiment. It involves controls, including a potato juice and hydrogen peroxide reaction, in different test tubes. The experiment details the measurement and recording of results.
Full Transcript
Positive and negative controls (Experiment 1) Experimental procedures 1. Obtain and label three 15 ml conical plastic reaction tubes. 2. Then to Tube 1 Add 1 ml of potato juice (catalase) to tube 1 (use a plastic transfer pipette). Add 4 ml of hydrogen peroxide to tube 1. Swirl well to mix and wait...
Positive and negative controls (Experiment 1) Experimental procedures 1. Obtain and label three 15 ml conical plastic reaction tubes. 2. Then to Tube 1 Add 1 ml of potato juice (catalase) to tube 1 (use a plastic transfer pipette). Add 4 ml of hydrogen peroxide to tube 1. Swirl well to mix and wait at least 20 seconds for bubbling to develop. Use a ruler and measure the height of the bubble column above the liquid (in millimeters; use the centimeter scale of the ruler) and record the result in Table ----3. Then to Tube 2 Add 1 ml of water. Add 4 ml of hydrogen peroxide. Swirl well to mix and wait at least 20 seconds. Measure the height of the bubble column (in millimeters) and record the result in 4. Then to Tube 3 Add 1 ml of potato juice (catalase). Add 4 ml of sucrose solution. Swirl well to mix; wait 20 seconds. Measure the height of the bubble column and record the result in Table --- Untitled 1
