Central Dogma PDF

The Central Dogma Copyright and Disclosure All materials found on GCSoM’s course and project sites may be subject to copyright protection and are restricted from further dissemination, retention, or copying. I have no financial relationship with a commercial entity producing health care related pro...

The Central Dogma Copyright and Disclosure All materials found on GCSoM’s course and project sites may be subject to copyright protection and are restricted from further dissemination, retention, or copying. I have no financial relationship with a commercial entity producing health care related products and/or service.  Learning Objectives describe phases of the central dogma explain the purpose and products of each phase  The Central Dogma (Eukaryotes) DNA replication reverse transcription Shafee T, Lowe R (2017). "Eukaryotic and prokaryotic gene structure". WikiJournal of Medicine 4 (1). DOI:10.15347/wjm/2017.002. ISSN 20024436 3 DNA Replication https://www.genome.gov/genetics-glossary/DNA-Replication 4 Transcription: Eukaryotic Promoter/Enhancer Region Transcriptional Repressor Transcriptional Co- Transcription activators General TFs and RNA Polymerase RNA CAAT TATA Silencer Enhancer ( ~ -70 to -80) Hogness box (~ -25) “response Promoter elements” Region 5 RNA Processing: Pre-mRNA and mRNA Structure CAP Introns Exons polyA site 5’ss 3’ss 5’ UTR splice sites of intron 3’ UTR CAP AAAAAAAAAAAAAAA…n polyA tail 6 Translation: Protein Synthesis the genetic code that is first transcribed into the mRNA subsequently, the mRNA is translated into proteins by ribosomes http://en.wikipedia.org/wiki/File:Peptide_syn.png 7 Author attribution: Boumphreyfr  Putting It Together the central dogma describes the flow of information from DNA to RNA to protein the steps (phases) include DNA replication, RNA transcription, RNA processing (and post-transcription modification in eukaryotes), and translation in reverse transcription, the informational flow is from RNA to DNA in general, the information flow transmits the data stored in the DNA’s genetic code into proteins that perform the functions of the cell 8