General Microbiology BY4013 Lecture Notes PDF

1 General Microbiology BY4013 Introduction Lecturer: Prof. Achim Schmalenberger, SR2-004 TAs: Rebecca Brennan Dean Brennan Liz Russell 1 2...

1 General Microbiology BY4013 Introduction Lecturer: Prof. Achim Schmalenberger, SR2-004 TAs: Rebecca Brennan Dean Brennan Liz Russell 1 2 Purpose of todays 1st lecture Introduce the course and topics Information on where to get information! Assessment of course Things that you need Very short history in microbiology 2 1 3 Learning outcomes A) Cognitive Appreciate the history and evolution of the science of microbiology Define and differentiate the fundamental characteristics of major groups of bacteria and viruses Understand microbiological classification and nomenclature Recognize the key requirements for bacterial growth Critically evaluate methods for control of microbial growth Discuss the significance of microbes in biotechnology, the environment and disease The prokaryotic cell 3 4 Learning outcomes B) Psychomotor Perform basic microbiological such as aseptic techniques Manipulate bacterial cultures Detect and quantify the presence of specific microbes in water and/or food samples Streak plate 4 2 5 General Microbiology comprises lectures and labs 2h lecture slot per week – Friday 2-4pm DG16 – Lecture slides will be available week by week on Brightspace Physical labs (2h), Wednesdays, week 2-10 All students need to prepare for the labs in advance; read the manual, take notes, watch the videos, to be ready to work independently Take the online quiz in order to be able to join the lab You need to pass the lab quiz for labs 1-6 5 6 Lecture topics Topic 1 Brief History of microbiology Topic 2 Working with microbes Topic 3 Eukaryotic microorganisms Topic 4 Structure of bacterial cells Topic 5 Bacteria cell features and spores Topic 6 Bacterial taxonomy Topic 7 Control of microbial growth Topic 8 Microbial physiology, nutrition and growth Topic 9 Microbial ecology 1+2 6 3 7 Lecture topics Topic 10 Wastewater Topic 11 Water and health Topic 12 Viruses and prions Topic 13 Food microbiology Topic 14 Commensals Topic 15 Infection and immunology Topic 16 Microbial diseases Topic 17 Antibiotics and resistance Topic 18 Investigating microbes on nucleic acid basis 7 8 Aside from the acquisition of knowledge - Why do you need BY4013? As a basis for – Underlying basis for all biological processes on earth – Teaching biology to leaving cert in schools (Science Ed.) – Understanding equine diseases (Equine Sci.) – Understanding environmental processes (Environmental Sci.) – Understanding fermenting processes (Industrial Biochem.) – Understanding (food-borne) pathogens (Food & Health, Bioscience) – Subsequent modules Microbiology and Immunology, Microbial Technology 2, Food Microbiology , Host Microbe Interactions. – Fourth year project opportunities 8 4 9 Labs Start week 2 in the Health Sciences Building HS1014 &1006 Compulsory, unless sick or infectious (provide evidence) Manual will be available on Brightspace print it out/collect from print room? – Read the introduction before attending your first lab – Study the experimental section before attending the relevant lab, watch the videos – Take the quiz before coming to the lab (labs 1-6) – Don’t forget your lab coat, goggles, marker pen, facemask if necessary – General use of mobile phones is not permitted during lab sessions Will follow lecture topics 9 10 Labs Return to the same session and the same bench location for each lab Moving labs as well as places within the lab are not permitted We work in pairs, do not swap lab partners Masks can be a hazard when set on fire  Use FFP2/KN95/N95 masks, the latter have class 1 flammability (= normal flammability)  Do NOT use cloth or surgical masks, they are more flammable 10 5 11 Where to get info Brightspace: BY4013 – Lecture notes and lab manuals (PDF) – Several lab procedures pre-recorded available on media.heanet.ie Books: Reading list on Brightspace – Madigan et al.: Brock Biology of Microorganisms 5 short loan books of 12th edition in library 13th and 14th edition available in library 15th & 16th edition (2019) available online – Essential Microbiology by Stuart Hogg, 2nd edition 2013 – Microbiology books by Prescott et al.;  Look up index & read relevant paragraph(s). Secondary materials in “Module Material” folder I will use Brightspace messages to inform class about issues 11 12 BY4013 assessments: Assessment Assignments Activities Submission Due Value [100%] Opens Assignment 1 Mid-term 1 (Brightspace) Week 6 Week 6 10 Assignment 2 Mid-term 2 (Brightspace) Week 11 Week 11 10 Lab preparation quizzes Assignment 3 See Brightspace See Brightspace Pass/fail (Brightspace) Lab Reports (4) Assignment 4 (Brightspace) & Bacterial See Brightspace See Brightspace 16 + 4 Identification (lab) Assignment 5 Streak test (lab) Week 11 Week 11 10 Assignment 6 Final exam (on site) Week 14-15 50 Continuous assessment: Late submissions: 10% deduction per day in case no valid excuse (e.g. illness with cert) has been provided. 12 6 13 Grading scheme for BY4013 Biological Science grading (since 2013) Grade Marks % A1 >85 A2 80-84.9 B1 75-79.9 B2 70-74.9 B3 65-69.9 C1 60-64.9 C2 55-59.9 C3 50-54.9 D1 45-49.9 D2 40-44.9 F