Blood Test Tube Collection and Waste Management PDF

Sample Collection and Waste Management Lab 1  Specimen Collection Why specimen collection is important? Specimen collection and transportation are critical considerations, because any results the laboratory generates is limited by the quality of the...

Sample Collection and Waste Management Lab 1  Specimen Collection Why specimen collection is important? Specimen collection and transportation are critical considerations, because any results the laboratory generates is limited by the quality of the specimen and its condition upon arrival in the laboratory. specimens should be obtained properly to minimize the possibility of introducing contaminating microorganisms that are not involved in the infectious process.  Containers and swab for the collection of specimens Containers : for feces:- - Universal container Spoon attached to the inside of the screw cap  For urine:- - Universal container for small quantities. - For larger quantities 250ml wide mouthed screw-capped bottles are convenient. For sputum:- - Universal container should not be used, wide mouthed disposable containers should be used  For blood:- - Without anticoagulant for serological examination. - With EDTA for parasitological examination. ✓Blood culture bottle - This must be at least large enough to hold 50ml of liquid medium, with which it is issued from laboratory, plus 5-10ml of patient’s blood.  Syringe and needle for aspiration - Wound pus - CSF - Pleural effusion - Amniotic fluid - Synovial fluid  Swabs:- Swabs are suitable for taking specimens for exudates from the throat, nose, ear, skin, wounds and other accessible lesions. It consists of sterile pledge of absorbent material, usually cotton-wool or synthetic fiber, mounted on a thin wire stick. ❖ Swabs for special purpose: ✓ Baby swabs ✓ Pre-nasal swabs ✓ Post-nasal swabs ✓ Laryngeal swabs ✓ High vaginal and cervical swabs  Specimen collection guidelines Time of collection 1. During the acute phase 2. Before antimicrobial therapy 3. Time of the day (1st morning) Contamination - Normal flora Specimen containers - sterile, leak proof  Labeling specimen Each sample must have a label attached to the specimen container bearing the following information: 1. Patient’s name 2. Type of specimen 3. Collection date and time 4. Test requested 5. Name of ordering physician  Specimen rejection criteria Unlabeled or improperly labeled specimen Mismatch information Improper container (non sterile) Improper temperature Insufficient specimen quantity Leaking container Dried out swab Late specimen ( more than 2hrs and not preserved) Physician must be informed about rejection