BIOT 202 Week 12 Class - Tagged.pdf

BIOT 202 – Molecular Biology Lecture Objectives Enzymes Naming Enzymes Lab Application: Restriction Digests with Endonucleases and Ligation with Ligase Lab Application: Identifying a Protein Using a Protease (more on this next week) Lecture Objectives Spontaneous vs Non-spontaneous Re...

BIOT 202 – Molecular Biology Lecture Objectives Enzymes Naming Enzymes Lab Application: Restriction Digests with Endonucleases and Ligation with Ligase Lab Application: Identifying a Protein Using a Protease (more on this next week) Lecture Objectives Spontaneous vs Non-spontaneous Reactions Spontaneous vs Non-spontaneous Reactions Spontaneous Reaction Activation Energy Metastable State Transition State Must Overcome Activation Energy Lecture Objectives Catalysts Catalysts Enzymes as Catalysts Enzymes as Catalysts Lecture Objectives Enzymes Enzymes as Biological Catalysts Lab Application: Testing for Enzyme Presence Active Site of an Enzyme Enzyme Specificity Enzyme Specificity Enzyme Specificity Enzyme Form and Function Lock-and-Key Model Induced Fit Model Induced Fit Model Enzyme Catalyzed Reactions Example of an Enzyme Catalyzed Reaction Isoenzymes Temperature and Enzyme Activity Temperature and Rate of Growth pH and Enzyme Activity pH and Enzyme Activity Optimum pH for Selected Enzymes Lecture Objectives Enzyme kinetics How Can Reaction Rate Be Increased? Enzyme Concentration and Reaction Rate Substrate Concentration and Reaction Rate Reaction Velocity and Substrate Concentration Enzyme Catalyzed Reaction Rate Enzyme Catalyzed Reaction Rate Reaction Calculations for Catalytic Efficiency Michealis-Menten Equation Lineweaver-Burke Plot Reaction Calculations for Catalytic Efficiency Lecture Objectives Denaturing Enzymes Enzyme Inhibitors Enzyme Inhibitors Reversible Enzyme Inhibitors Reversible Competitive Inhibitor Example of a Competitive Inhibitor Reversible Non-competitive Inhibitor Irreversible Enzyme Inhibitors Irreversible Enzyme Inhibitors Other Methods of Enzyme Regulation Allosteric Regulation Covalent Regulation Lecture Objectives