Bacterial Smear and Simple Staining Procedure PDF

SMEAR PREPARATION AND SIMPLE STAINING CRISELDA DANG S. VISTA, RMT, MPH (c) LABORATORY OBJECTIVES Learn the proper procedure for smear preparation Know the purpose of staining in identifying bacteria Identify the different forms and shapes of bacteria using simple...

SMEAR PREPARATION AND SIMPLE STAINING CRISELDA DANG S. VISTA, RMT, MPH (c) LABORATORY OBJECTIVES Learn the proper procedure for smear preparation Know the purpose of staining in identifying bacteria Identify the different forms and shapes of bacteria using simple staining technique SMEAR PREPARATION Performed to visualize the presence of bacteria The specimen is spread thinly on a glass microscope slide and allowed to air-dry The film should be thin enough that individual bacterial cells can be seen Bacteria should not be washed off during staining Bacterial forms should not be distorted SMEAR PREPARATION IF THE MATERIAL TO BE EXAMINED IS A LIQUID: Transfer using a sterile swab, pipette, or inoculating loop and spread directly on the dry slide SMEAR PREPARATION IF THE SAMPLE IS AN ISOLATED COLONY: Place a drop of sterile water into the glass slide to suspend the bacteria; then spread over the slide SMEAR PREPARATION The material or All slides prepared must be specimen should be spread labeled with sufficient thinly but evenly over the patient identification middle area of the slide information Air-dry the material Fix the slide STAINS Are chemical substances that contain dyes. Certain bacterial structures have affinities for particular dyes. Special stains - useful for identifying the morphology of bacteria and visualizing structures such as capsules, flagella, spores, and granules. Simple stains - used to identify cellular details SIMPLE STAINING Single chemical stain is used All structures present are stained one color Organisms should be observed for size, shape, cellular arrangement, and uniformity of staining BACTERIAL SHAPE AND ARRANGEMENTS DIPLOCOCCI - cocci in pairs COCCI  COCCI Streptococci- cocci in chains COCCI Staphylococci- irregular clumps or clusters COCCI Tetrads- cocci in packets of 4 Sarcinae- cocci in packets of 8,16,32 cells COCCI BACILLI Cocobacilli - small round rods BACILLI Comma, S, Spiral shaped rods BACILLI Straight, rod shaped  BACILLI Spore forming rods (Clostridium, Bacillus) Mold-like filamentous BACILLI