ANAPHY LAB NOTES - Google Docs.pdf

‭Professor Josh Macapallag‬ ‭ANAPHY‬ ‭1-CMT-1B‬ ‭3.‬ R ‭ evolving‬ ‭nosepiece‬ ‭-‬ ‭circular‬ ‭piece‬ ‭that‬ ‭L1: MICROSCOPE BASICS‬...

‭Professor Josh Macapallag‬ ‭ANAPHY‬ ‭1-CMT-1B‬ ‭3.‬ R ‭ evolving‬ ‭nosepiece‬ ‭-‬ ‭circular‬ ‭piece‬ ‭that‬ ‭L1: MICROSCOPE BASICS‬ ‭allows rotation of different objective lenses‬ ‭4.‬ ‭Arm - used for transporting the microscope‬ ‭5.‬ ‭Stage - the flat surface below the objective.‬ ‭The microscope‬ ‭6.‬ ‭Iris‬ ‭diaphragm‬ ‭-‬ ‭like‬ ‭an‬ ‭eye,‬ ‭it‬ ‭controls‬‭the‬ ‭★‬ ‭From the Greek work‬‭microscopium‬ ‭amount‬ ‭of‬ ‭light‬ ‭that‬ ‭passes‬ ‭through‬ ‭the‬ ‭★‬ ‭Mikros -‬‭small ;‬‭Skopein -‬‭look at‬ ‭bottom of the stage‬ ‭★‬ ‭Instrument of viewing‬ ‭7.‬ ‭Coarse‬ ‭adjustment‬ ‭knob‬ ‭-‬ ‭used‬ ‭for‬ ‭quick‬ ‭★‬ ‭Allowed‬ ‭scientists‬ ‭to‬ ‭make‬ ‭new‬‭insights‬‭on‬ ‭initial focusing. The bigger between the two‬ ‭the body and diseases‬ ‭8.‬ ‭Fine‬ ‭adjustment‬ ‭knob‬ ‭-‬ ‭used‬ ‭to‬ ‭move‬ ‭the‬ ‭stage‬ ‭closer‬ ‭slowly‬ ‭for‬ ‭precise‬ ‭focus.‬ ‭The‬ ‭Notable figures:‬ ‭smaller between the two‬ ‭1.‬ ‭Zacharias Janssen‬ ‭9.‬ ‭Light‬ ‭source‬ ‭-‬ ‭provides‬ ‭light‬ ‭for‬ ‭the‬ ‭-‬ ‭created‬ ‭one‬ ‭of‬ ‭the‬ ‭earliest‬ ‭compound‬ ‭microscope‬ ‭microscopes (1600)‬ ‭10.‬‭Base‬ ‭-‬ ‭a‬ ‭square-shaped‬ ‭support‬ ‭for‬ ‭2.‬ ‭Antonie Van Leeuwenhoek‬ ‭transporting the microscope‬ ‭-‬ ‭made‬ ‭simple‬ ‭microscopes‬ ‭with‬ ‭his‬ ‭own‬ ‭11.‬‭Objective lenses‬ ‭lenses.‬ ‭★‬ ‭Scanning (4x) - initial location‬ ‭- observed animal, blood, and sperm cells‬ ‭★‬ ‭Low‬‭power‬‭(10x)‬‭-‬‭initial‬‭location,‬‭or‬ ‭3.‬ ‭Robert Hook‬ ‭for‬ ‭observing‬ ‭specimens‬ ‭that‬ ‭don’t‬ ‭-‬ ‭published‬ ‭Micrographia,‬ ‭copper-plate‬ ‭need high magnification‬ ‭illustrations.‬ ‭★‬ ‭High‬ ‭power‬ ‭(40x,‬‭43x,‬‭45x)‬‭-‬‭40x‬‭is‬ ‭-‬ ‭saw‬ ‭pores‬ ‭in‬ ‭a‬ ‭cork‬ ‭that‬ ‭resembled‬ ‭commonly‬ ‭used,‬ ‭while‬ ‭43x‬ ‭and‬ ‭45x‬ ‭honeycombs‬ ‭are‬ ‭considered‬ ‭high-dry‬ ‭powers‬ ‭-‬ ‭credited‬ ‭for‬ ‭naming‬ ‭the‬ ‭cell‬‭,‬ ‭the‬ ‭building‬ ‭(does not need oil)‬ ‭blocks of life‬ ‭★‬ ‭Oil‬ ‭immersion‬ ‭(100x)‬ ‭-‬ ‭used‬ ‭for‬ ‭extremely‬ ‭small‬ ‭specimens‬ ‭such‬ ‭as‬ ‭Main component:‬‭Compound light microscope‬ ‭bacteria‬ ‭and‬ ‭blood‬ ‭cells.‬ ‭It‬ ‭needs‬ ‭★‬ ‭Has‬ ‭compound‬ ‭lenses‬ ‭that‬ ‭magnify‬‭objects.‬ ‭cedar wood oil to function.‬ ‭It bends or refracts light‬ ‭Parts of the microscope‬ ‭1.‬ ‭Eyepiece‬‭-‬‭also‬‭known‬‭as‬‭the‬‭ocular‬‭lens‬‭,‬‭is‬ ‭located‬ ‭at‬ ‭the‬ ‭top‬ ‭of‬ ‭the‬ ‭microscope‬ ‭and‬ ‭is‬ ‭responsible for magnifying an object‬ ‭2.‬ ‭Body‬ ‭/‬ ‭eye‬ ‭/‬ ‭ocular‬ ‭tube‬ ‭-‬ ‭cylindrical‬‭piece‬ ‭that‬ ‭connects‬ ‭the‬ ‭eyepiece‬ ‭to‬ ‭the‬ ‭objective‬ ‭lens. It holds the ocular lens in place‬