Types of Media for Growing Bacteria PDF
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This document discusses types of media used for growing bacteria in a laboratory setting. It covers general, specialized, enriched, defined, complex, non-selective, selective, enrichment, differential, and combined media. Understanding these media types is essential for successful microbiological experiments.
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Types of Media for Growing Bacteria: General All-Purpose Media: ○ Supports many types of organisms. ○ Example: Tryptic Soy Broth (TSB). Specialized Media: ○ Used for identifying bacteria. ○ Contains dyes, pH indicators, or antibiotics. E...
Types of Media for Growing Bacteria: General All-Purpose Media: ○ Supports many types of organisms. ○ Example: Tryptic Soy Broth (TSB). Specialized Media: ○ Used for identifying bacteria. ○ Contains dyes, pH indicators, or antibiotics. Enriched Media: ○ Contains nutrients, vitamins, and growth factors. ○ Supports "fastidious" organisms (those that need extra nutrients). Chemically Defined Medium: ○ All components are known and specified. ○ Example: EZ medium. Complex Media: ○ Made from extracts of yeast, meat, or plants. ○ Exact composition is unknown. ○ Examples: Nutrient broth, Tryptic Soy Broth, Brain Heart Infusion. Non-Selective Media: ○ Supports growth of all microorganisms. ○ Example: Mueller-Hinton agar, used for antibiotic testing. Selective Media: ○ Inhibits unwanted microorganisms while supporting specific ones. ○ Example: MacConkey agar, which favors gram-negative bacteria. Enrichment Cultures: ○ Promote growth of a specific microorganism from a mixture. ○ Example: Culturing oil-eating bacteria with crude oil as the only carbon source. Differential Media: ○ Allows for easy identification of different bacteria by color changes. ○ Example: MacConkey agar shows color change for lactose fermenters. Combined Media: ○ Selective and differential media can be used together for better bacterial identification.
