Specimen Collection for Microbiology Studies PDF

Specimen Collection for Microbiological Studies Dr. Basma Samir (MD) Types of Biological Specimens 2 SPUTUM WOUND URINE TISSUE FAECES BODY FLUIDS BLOOD BRONCHIAL / WASH / LAVAGE BONE MARROW...

Specimen Collection for Microbiological Studies Dr. Basma Samir (MD) Types of Biological Specimens 2 SPUTUM WOUND URINE TISSUE FAECES BODY FLUIDS BLOOD BRONCHIAL / WASH / LAVAGE BONE MARROW THORAT SWAB PUS EAR EYE SPUTUM 3 Bacteria 4 5 Sputum Assure patient cooperation to get an adequate specimen. Instruct the patient as follows: Rinse mouth with clean water to remove food particles and debris. Have patient breathe deeply and cough several times to achieve a deep specimen. Patient should expectorate into dry, sterile container. Transport immediately at ambient temperature. Refrigerate if a delay of more than one hour is anticipated.  Sputum 7 Expectorated sputum is acceptable for bacterial, mycobacterial, and fungal cultures. Microbiology Lab may reject samples for bacterial culture that are not indicative of deeply expectorated specimens. Sputum Patients with clinical and chest x-ray findings compatible with TB should collect: 3 first early morning sputum specimens (preferably on 3 separate days) for AFB culture. into a sterile container with lid sealed tightly and transported to the laboratory in double sealed bags. Induced Sputum Sputum induction is a procedure to help a patient cough up secretions from the lungs more easily. The principle is to create extra moisture in the airways of the lungs. This will loosen and thin out secretions so they may be coughed up more easily. Induced Sputum The patient inhales nebulized hypertonic saline solution, which liquefies airway secretions, promotes coughing and allows expectoration of respiratory secretions. Sputum induction is simple and non-invasive. The procedure produces coughing so it is likely that infectious droplets, if present, will be expelled into the room air. Strict airborne respiratory precautions should be observed whenever sputum induction is performed. 11 Urine for Bacterial, Fungal, AFB Cultures Instructions to collect midstream urine for bacterial culture: Wash hands thoroughly with soap and water, rinse and dry. Wash urinary opening and surrounding area. Take the open sterile cup in the other hand without touching the rim or inner surface of the cup or lid. Void 20 to 25 ml into the toilet and catch a portion of the rest of urine in the container without stopping the stream. Place the lid securely on the cup. Immediately transport to the lab, refrigerate in case of delay.  Indwelling catheter urine: Do not collect urine from the drainage bag because growth of bacteria outside the catheter may have occurred at this site. Clean the catheter with an alcohol pad. Use a sterile needle and syringe to puncture the tubing. Aspirate urine directly from the tubing. Transfer the urine to a sterile specimen container. Immediately transport to the lab, refrigerate in case of delay.  For AFB CULTURE: Entire first morning Urine specimen should be collected, on minimum three Consecutive days, in specially provided sterile containers Stool, Faeces Collect specimen in a clean bed pan or use plastic wrap placed between the toilet seat and the bowl. Do not submit feces contaminated with urine or toilet water. Transfer specimen into a clean, dry container or the appropriate preservative. Transport at ambient temperature within two hours of collection.