OCR A-Level Biology 2.2 Biological Molecules PDF

OCR (A) Biology A-level Topic 2.2: Biological molecules Notes www.pmt.education  Water Water is a very important molecule which is a major component of cells, for instance: Water is a polar molecule due to uneven dis...

OCR (A) Biology A-level Topic 2.2: Biological molecules Notes www.pmt.education  Water Water is a very important molecule which is a major component of cells, for instance: Water is a polar molecule due to uneven distribution of charge within the molecule – the oxygen atom attracts electrons a bit more strongly than the hydrogen atoms. The unequal sharing of electrons gives the water molecule a slightly negative charge near its oxygen atom and a slight positive charge near its hydrogen atoms. It is a metabolite in metabolic reactions such as condensation and hydrolysis which are used in forming and breaking of chemical bonds It is a solvent in which many metabolic reactions occur It has a high heat specific capacity meaning that a lot of energy is required to warm water up therefore minimising temperature fluctuations in living things therefore it acts as a buffer It has a relatively large latent heat of vaporisation, meaning evaporation of water provides a cooling effect with little water loss Strong cohesion between molecules enables effective transport of water in tube-like transport cells as the strong cohesion supports columns of water. As a result of strong cohesion the surface tension at the water-air boundary is high Maximum density of water is at 4 degrees – this means that ice is less dense than water and floats on top of it creating an insulating layer, this increases the chance of survival of organisms in large bodies of water as it prevents them from freezing Water is incompressible therefore it provides good support Monomers and polymers Monomers are small units which are the components of larger molecules, examples of include monosaccharides (such as glucose), amino acids and nucleotides. Polymers are molecules made from monomers joined together. A condensation reaction is a reaction which joins monomers by chemical bonds and it involves the elimination of a water molecule. Hydrolysis is the opposite of condensation and it’s when water is added to break a chemical bond between two molecules. www.pmt.education  Carbohydrates Carbohydrates are molecules which consist only of carbon, hydrogen and oxygen and they are long chains of sugar units called saccharides. There are three types of saccharides - monosaccharides, disaccharides and polysaccharides. Monosaccharides can join together to form disaccharides and polysaccharides by glycosidic bonds which are formed in condensation reactions. Monosaccharides Glucose is a monosaccharide containing six carbon atoms in each molecule, it is the main substrate for respiration therefore it is of great importance. It has two isomers – alpha and beta glucose with following structures: Figure 1 A dash of science Disaccharides: Maltose is a disaccharide formed by condensation of two glucose molecules Sucrose is a disaccharide formed by condensation of glucose & fructose Lactose is a disaccharide formed by condensation of glucose & galactose Polysaccharides are formed from many glucose units joined together and include: Glycogen and starch which are both formed by the condensation of alpha glucose Cellulose formed by the condensation of beta glucose Glycogen is the main energy storage molecule in animals and it’s formed from many molecules of alpha glucose joined together by 1, 4 and 1, 6 glycosidic bonds. It has a large number of side branches meaning that glucose & therefore energy, can be released quickly. Moreover, it is a relatively large but compact molecule thus maximising the amount of energy it can store. Starch stores energy in plants and it is a mixture of two polysaccharides called amylose and amylopectin: Amylose – amylose is an unbranched chain of glucose molecules joined by 1, 4 glycosidic bonds, as a result of that amylose is coiled and thus it is a very compact molecule meaning it can store a lot of energy Amylopectin is branched and is made up of glucose molecules joined by 1, 4 and 1, 6 glycosidic bonds, due to the presence of many side branches it is rapidly digested by enzymes therefore energy is released quickly. www.pmt.education Cellulose is a component of cells wells in plants and it’s composed of long, unbranched chains of beta glucose which are joined by glycosidic bonds. Microfibrils are strong threads which are made of long cellulose chains joined together by hydrogen bonds and they provide structural support in plants cells. Lipids Lipids are biological molecules which are only soluble in organic solvents such as alcohols. There are two types of lipids: Saturated lipids such as those found in animal fats – saturated lipids don’t contain any carbon-carbon double bonds Unsaturated lipids which can be found in plants – unsaturated lipids contain carbon-carbon double bonds and melt at lower temperatures than saturated fats. Figure 2 Spark People The greater the number of unsaturated bonds, the weaker the intermolecular bonds resulting in lower melting point, and as a result of that saturated fats which don’t contain any double bonds are solid at liquid temperature and unsaturated lipids are liquid at room temperature. Triglycerides are lipids made of one molecule of glycerol and three fatty acids joined by ester bonds formed in condensation reactions. There are many different types of fatty acids, they vary in chain length, presence and number of double bonds. Also, some triglycerides contain a mix of different fatty acids. Triglycerides are used as energy reserves in plant and animal cells. Figure 3 The Med Circle In phospholipids, one of the fatty acids of a triglyceride is substituted by a phosphate- containing group. Phosphate heads are hydrophilic and the tails are hydrophobic and as a result phospholipids form micelles when they are in contact with water as heads are on the outside as they are attracted to water and tails are on the inside as they move away from water. www.pmt.education  Inorganic ions Inorganic ions occur in solution in the cytoplasm and body fluid of organisms, some in high concentrations and others in very low concentrations. Some of the essential ions include: hydrogen ions which determine the pH of substances such as blood – the higher the concentration of hydrogen ions the lower the pH Iron ions are a component of haemoglobin which is an oxygen carrying molecule in red blood cells Sodium ions are involved in co-transport of glucose and amino acids Phosphate ions are a component of DNA and ATP Proteins Amino acids are the monomers from which proteins are made. Amino acids contain an amino group – NH2, carboxylic acid group and a variable R group which is a carbon-containing chain. There are 20 different amino acids with different R groups. Amino acids are joined by peptide bonds formed in condensation reactions. A dipeptide contains two amino acids and Figure 4 Geography Discipline Network polypeptides contain three or more amino acids. Structure of proteins is determined by the order and number of amino acids, bonding present and the shape of the protein: Primary structure of a protein is the order and number of amino acids in a protein. The secondary structure is the shape that the chain of amino acids takes – either alpha helix or beta pleated sheet. The shape is determined by the hydrogen bonding. Tertiary structure of proteins is the 3D shape of the protein.It can be globular or fibrous. Globular proteins such as enzymes are compact whereas fibrous proteins such as keratin are long and thus can be used to form fibres. For instance, collagen is a fibrous protein of great strength due to presence of both hydrogen and covalent bonds in the structure. Collagen molecules wrap around each other and form fibrils which form strong collagen fibres. Collagen forms the structure of bones, cartilage and connective tissue and is a main component of tendons which connect muscles to bones. www.pmt.education  Haemoglobin is a water soluble globular protein which consists of two alpha and two beta polypeptide chains each containing a haem group. It carries oxygen in the blood as oxygen can bind to the haem (Fe2+) group and oxygen is then released when required. Tests Benedict’s test for reducing and non-reducing sugars - a reducing sugar is one which can donate electrons and includes all monosaccharides and some disaccharides. An alkaline solution of blue copper (II) sulphate is added to the sugar and subsequently heated, in the presence of reducing sugar, a red precipitate of copper (I) oxide is formed. In the absence of reducing sugar, the Benedict’s reagent does not change colour. The biuret test is used to test for proteins. Firstly, a sample of the solution is placed in a test tube and subsequently an equal amount of NaOH is added. Afterwards, a few drops of dilute copper (II) sulfate solution is added and gently mixed. In the presence of a protein, the solution turns lilac/ as an indicator of peptide bonds. In the absence of protein, the solution remains blue. The emulsion test is used to test for lipids. Firstly, 2ml of sample is added to 5ml of ethanol. Subsequently, the contents are mixed thoroughly by shaking to ensure that the lipids dissolved. Upon addition of 5ml of water and mixing, the solution turns cloudy indicating the presence of lipid due to the formation of an emulsion where the light is refracted as it passes from droplets of oil to droplets of water. Starch is tested for with the help of iodine – in the presence of starch, the colour of potassium iodide solution from yellow to black/blue. www.pmt.education