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IMMUNOHEMATOLOGY by Elizabeth F. Williams Donor Selection and Blood Collection REMEMBER!...

IMMUNOHEMATOLOGY by Elizabeth F. Williams Donor Selection and Blood Collection REMEMBER! COLLECTION OF DONOR BLOOD 1. Registration, medical history and Danny physical examination (See Danny Donor) Donor 2. Aseptic technique, scrub site for minimum 30 seconds- providine-iodine scrub pt' GENERAL INFORMATION must be 1. Maximum collection - no more than normal; 10.5 mL of whole blood per kilogram of number body weight, including samples institution 2. Donor interval- 8 weeks after WB ~ defined donation...:;"'J.~o ~ 3. MD must evaluate medications 4. Medications taken within 48 hrs that irreversibly affect platelet fun ction (i.e., aspirin) may not he u sed as the only source for platelets but can be part of a platelet pool Criteria for Allogeneic Donor Selection Age ~ 16 yrs. or conform to state law no max (evaluate b M.D.) Temperature (oral) _s:37.5'C or.s: 99.S'F must e "wit in norma i,.m..~ its_._ "· ___.,....__ ~\I\ Blood Pressure no longer defined by AABB as 180/100; ~Ii institution defined. ~~ Hgb/ Hct ~12.5g%/ 38%~ t....--1::- -..::::::::...,__ _-+----- - - - - - - ; 1P" Weight minimum 110 lb / 50 kg 'f-..v# TEST REMEMBER! Blood Pressure Evaluate Suitability ofPotential Donor Systolic/ Diastolic Sky is above Dirt 2 Donor Deferral 1 YEAR: Hepatitis B Immune Globulin Possible exposure to hepatitis, Possible exposure to hepatitis, HIV & malaria HIV or malaria; Recipient of blood/blood products Tattoo - unless at state regulated facility Is living with or having sexual contact with a per- son positive for HBsAg or HBV NAT; is sympto- matic for hepatitis C of any other viral hepatitis Mucous membrane exposure to blood Skin penetration with instruments contaminated with blood/body fluid Sexual contact with individual symptomatic for any viral hepatitis, confirmed + for HBsAg / HIV or in high risk category From completion of therapy for syphilis or gonorrhea or reactive STS Traveled to endemic area for malaria > 72 hours in a correctional institution Asymptomatic during the time 3 YEAR: - Visitor/immigrant from area endemic for Possible exposure to malaria malaria - Previously diagnosed with malaria VIRAL DISEASES: INDEFINITE/ PERMANENT Viral hepatitis after age 11 Definite disease or habits strongly Confirmed positive test for HBsAg or positive associated with bloodborne HBV NAT result pathogens Repeatedly reactive test for anti-HBc or anti-HTLV Donated only unit to recipient who developed post transfusion hepatitis, HIV, or HTLV Present/Past infection of HCV, HTLV, HIV or T. cruzi Evidence of parenteral drug use OTHER DISEASES: Family history of CJD or risk of vCJD History of babesiosis COMMON BLOOD ANTICOAGULANTS, ADDITIVES AND REJUVENATING SOLUTIONS 1. Expiration with anticogulants and additives a. ACD/CPD/CPD2 - 21 days Donor Deferrals: b. CPDA-1 -35 days Focus on Bloodborne c. Additives- 42 Days Pathogens 2. Rejuvenating solutions a. Restores 2, 3-DPG and ATP b. Can freeze unit or, if used in 24 hrs. , can be stored at l-6C (must wash cells before transfusion to remove solution) KnowlecJse of Anticoagulants/Rejuvenating Solutions & Expiration Tim.es  3 AUTOLOGOUS DONATIONS Terms Associated with Apheresis 1. Donations for self; no age limit SEPARATION & COLLECTION OF 2. Hct ~ 33%; Hgb ~ 11 g/JL Cytapheresis cells 3. No bacteremia Plasmapheresis plasma 4. Collection > 72 hours prior to surgery or transfusion Plateletpheresis platelets Leu ka/Granulocytapheresis leukocytes/granulocytes 5. Autologous units - segregate from allogeneic units, only used for original c. Hematopoetic progenitor cells donor ❖ Can be collected from 6. Low volume collections peripheral circulation a. Use regular blood bags; volume drawn