Molecular Biology Transcription Class 17 Questions - Exam Questions PDF

Welcome to Class 17: Transcription Start with DNA replication, which IS on Exam 2 Transcription is NOT on exam 2 Check the syllabus for correct page ranges for readings https://www.inspiritvr.com/general-bio/ molecular-biology/rna-transc...

Welcome to Class 17: Transcription Start with DNA replication, which IS on Exam 2 Transcription is NOT on exam 2 Check the syllabus for correct page ranges for readings https://www.inspiritvr.com/general-bio/ molecular-biology/rna-transcription-study-guide 1) Antler dysplasia is an autosomal recessive genetic disease of jackalopes caused by a mutant allele of the Ant gene. The disease interferes with jackalopes’ ability to use their antlers to defend themselves from predators. However, heterozygous individuals (carriers of the mutant allele) have a survival advantage in hot desert climates (ie Arizona) because they radiate excess body heat through their antlers more efficiently. In temperate climates (ie Wisconsin), heterozygous and homozygous dominant individuals are equally fit. Which of the following statements is correct? a) Mutant alleles of the Ant gene will eventually disappear from populations in Wisconsin, but not Arizona. b) Carriers of the mutant Ant allele will make up the same percentage of the population in Wisconsin and Arizona. c) New mutations in the Ant gene will occur more frequently in Arizona than Wisconsin due to selective pressure. d) New mutations in the Ant gene will occur at the same rate in populations in Wisconsin and Arizona. 2) An F- E. coli cell is the recipient cell during conjugation. This cell acquires both antibiotic resistance and the ability to transfer the antibiotic resistance gene to other cells. The donor cell was most likely what kind of cell? a) F’ b) Hfr c) F+ d) Could have been either F+ or Hfr Khan Academy The sequence of newly synthesized RNA is A) Complementary to the coding strand and identical to the template strand B) Identical to the coding strand and complementary to the template strand C) Identical to both the coding and template strands D) Complementary to both the coding and template strands (“identical” with the exception that there will be U instead of T in RNA) The sequence of newly synthesized RNA is X A) Complementary to the coding strand and identical to the template strand B) Identical to the coding strand and complementary to the template strand X C) Identical to both the coding and template strands X Complementary to both the coding and template strands D) (“identical” with the exception that there will be U instead of T in RNA) If the sequence of the coding strand is 5' –CTGAT – 3' , what is the sequence of the corresponding mRNA? A) 5' – CUGAU – 3' B) 5' – UAGUC – 3' C) 5' – GACTA – 3' D) 5' – CTGAT – 3' If the sequence of the coding strand is 5' –CTGAT – 3' , what is the sequence of the corresponding mRNA? A) 5' – CUGAU – 3' X 5' – UAGUC – 3' B) X C) 5' – GACTA – 3' X D) 5' – CTGAT – 3' If the sequence of the template strand is 5' –ATCAG – 3' , what is the sequence of the corresponding mRNA? A) 5' – UAGUC – 3' B) 5' – TAGTC – 3' C) 5' – CUGAU – 3' D) 5' – CTGAT – 3' If the sequence of the template strand is 5' –ATCAG – 3' , what is the sequence of the corresponding mRNA? X 5' – UAGUC – 3' A) X 5' – TAGTC – 3' B) C) 5' – CUGAU – 3' X 5' – CTGAT – 3' D) Can you determine the sequence of a promoter by looking at the bases in mRNA? A) Yes B) No C) Maybe? 5) Can you determine the sequence of a promoter by looking at the bases in mRNA? X A) Yes Sequences that are upstream of the B) No transcription start site are not transcribed into RNA X Maybe? C) If you replaced some of the A:T base pairs in the -10 sequence with G:C base pairs, what might happen to the amount of RNA transcribed from that gene? A) Decrease B) Increase C) Stay the same If you replaced some of the A:T base pairs in the -10 sequence with G:C base pairs, what might happen to the amount of RNA transcribed from that gene? A) Decrease Increasing the proportion of G:C base pairs would make the DNA X Increase B) harder to unwind X Stay the same C) What is a change that the organism could make to counteract the effect of G:C base pairs making the DNA harder to unwind? What problem would the cell have if the -35 sequence of a promoter were changed drastically? The cell would not be able to: A) identify the beginning of the gene B) unwind the double helix to start transcription C) elongate the mRNA molecule once it had been started D) terminate transcription in a rho-independent manner 7) What problem would the cell have if the -35 sequence of the promoter were changed drastically? The cell would not be able to: A) identify the beginning of the gene X unwind the double helix to start transcription B) X elongate the mRNA molecule once it had been started C) X D) terminate transcription in a rho-independent manner What problem would the cell have if it did not have any sigma factor? The cell would not be able to: A) identify the beginning of genes B) unwind the double helix to start transcription C) elongate the mRNA molecule once it had been started D) terminate transcription in a rho-independent manner What problem would the cell have if it did not have any sigma factor? The cell would not be able to: A) identify the beginning of genes X unwind the double helix to start transcription B) X C) elongate the mRNA molecule once it had been started X D) terminate transcription in a rho-independent manner Which is a worse problem for the cell? A) A mutation in the -35 sequence of one gene B) A mutation in the gene encoding sigma factor C) They are both equally bad Which is a worse problem for the cell? X A mutation in the -35 sequence of one gene A) B) A mutation in the gene encoding sigma factor X They are both equally bad C) If the promoter sequence of the lacZ gene was deleted, how would DNA replication be affected? A) It would not be affected B) DNA replication would completely stop C) DNA replication of the lacZ gene would not be possible, but the rest of the genome would be okay If the promoter sequence of the lacZ gene was deleted, how would DNA replication be affected? A) It would not be affected X DNA replication would completely stop B) X C) DNA replication of the lacZ gene would not be possible, but the rest of the genome would be okay If all the helicases in the cell were deleted, which problem might occur? A) No problems, because transcriptional initiation does not use helicases B) Transcription would initiate, but not proceed to the end C) Some mRNAs would be too long If all the helicases in the cell were deleted, which problem might occur? X No problems, because transcriptional initiation does not use A) helicases X Transcription would initiate, but not proceed to the end B) C) Some mRNAs would be too long Which is the best consensus sequence? A) AAATGTGATNT B) AANTGTGANNN https://weblogo.berkeley.edu/examples.ht Which is the best consensus sequence? A) AAATGTGATNT X B) AANTGTGANNN https://weblogo.berkeley.edu/examples.ht