Chapter 5 Systems of Measurement, Laboratory Equipment, and Reagents PDF

Chapter 5 Systems of Measurement, Laboratory Equipment, and Reagents Preamble  PowerPoints are a general overview and are provided to help students take notes over the video lecture ONLY. ◦ PowerPoints DO NOT cover the details needed for the Unit exam  Each student is responsible...

Chapter 5 Systems of Measurement, Laboratory Equipment, and Reagents Preamble  PowerPoints are a general overview and are provided to help students take notes over the video lecture ONLY. ◦ PowerPoints DO NOT cover the details needed for the Unit exam  Each student is responsible for READING the TEXTBOOK for details to answer the UNIT OBJECTIVES  Unit Objectives are your study guide (not this PowerPoint)  Test questions cover the details of UNIT OBJECTIVES found only in your Textbook! 1  Systems of Measurement  Measuring accurately is the cornerstone of clinical laboratory analyses.  Different measurements worldwide ◦ Metric System – Traditional in the clinical lab; based on decimal system. ◦ International System (SI System) – Adopted in the 1960’s as a way to standardize measurements ◦ Common Base SI Units used in the laboratory  Meter (m)– measures length  Kilogram (kg)– measures mass  Second (s)- measures time  Mole (m)– amount of substance Systems of Measurement Most common prefixes of SI system in the Clinical Laboratory Deci- (d) 10-1 0.1 Centi-(c) 10-2 0.01 Milli-(m) 10-3 0.001 Micro-(u) 10-6 0.000001 2  Systems of Measurement  In the Clinical Laboratory, temperature is recorded based on the Celsius scale.  Temperature Conversations  To convert Fahrenheit to Celsius °C = (°F − 32) x 5/9 –or- °C = (°F − 32) / 9/5  To convert Celsius to Fahrenheit °F = (C° x 9/5) + 32 Systems of Measurement  Non-SI Units ◦ Several are used in the lab which include:  Minutes, hours, days – measure time  Liter (L)– measures volume; subdivided-mL & uL  Millimeters of mercury (mmHg)- measures pressure  International units (IU) – measures enzyme activity  Reporting Results in SI Units ◦ Lab results are reported as mass concentration unit – mass/liter(ex. g/dL). 3  Laboratory Glassware  Glassware is considered laboratory equipment  Many varieties of glass available. Each with a different function and method of use.  They are calibrated at 20oC Types of commonly used glass and their properties Kimax or Pyrex Relatively inert borosilicate glass. All-purpose. For use with 96% silicate; high resistance to heat temperatures up to 900° and cold shock C; also has good optical properties Corning Boron Soft, low-boron glass (