UFFELMANNS-TEST-1E-1F.pdf

UNP - College of Medicine - 1F & 1E UFFELMAN'S TEST Biochemistry FOR LACTIC ACID DETECTION LACTIC ACID FORMATION  Cellular FUNCTION OF Respiration LACTIC ACID Glucose Production Regulation of Inflammation Molecular...

UNP - College of Medicine - 1F & 1E UFFELMAN'S TEST Biochemistry FOR LACTIC ACID DETECTION LACTIC ACID FORMATION  Cellular FUNCTION OF Respiration LACTIC ACID Glucose Production Regulation of Inflammation Molecular Signaling  JULIUS UFFELMANN German physician REAGENT PREPARATION: 10mL of 1% Phenol + 1-2 drops of ferric chloride → IRON PHENOLATE  PRINCIPLE UFFELMANN’S REAGENT + LACTIC ACID → IRON LACTATE + PHENOL COLOR DEVELOPMENT: LACTIC ACID: YIELDS TO A LIGHT YELLOW OR YELLOW-GREEN COLOR The test can be used to detect the LACTIC presence of lactic ACIDOSIS: acid, helping to diagnose conditions that lead to lactic acidosis, such as sepsis or certain metabolic diseases.  LIMITATIONS It does not determine the concentration of lactic acid. It requires special precautionary measures. THANK YOU