Which enzyme is used in Gibson Assembly?
Understand the Problem
The question is asking which specific enzyme is used in the Gibson Assembly technique, which is a molecular biology method for assembling DNA fragments. The options provide different enzymes that may be involved in DNA manipulation.
Answer
T5 exonuclease, Phusion polymerase, and Taq ligase.
The enzymes used in Gibson Assembly are T5 exonuclease, Phusion polymerase, and Taq ligase.
Answer for screen readers
The enzymes used in Gibson Assembly are T5 exonuclease, Phusion polymerase, and Taq ligase.
More Information
Gibson Assembly is a method used to join multiple DNA fragments into a single, contiguous sequence. The combined activity of these three enzymes enables seamless insertion of DNA fragments by overlapping complementary sequences.
Tips
A common mistake is confusing the types of DNA polymerase and ligase used in the assembly process. It is essential to use compatible enzymes such as Phusion polymerase and Taq ligase.
Sources
- The web page with info on - Gibson Assembly® | NEB - neb.com
- Gibson Assembly Protocol - Addgene - addgene.org
- A Guide to Gibson Assembly Design - University of Warwick - warwick.ac.uk
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