What is the process of cloning genes in bacterial plasmids as shown in the image?
Understand the Problem
The question is likely asking for a summary or explanation of the cloning method depicted in the image related to producing clones of cells carrying recombinant plasmids. This involves several steps to isolate and manipulate DNA from a hummingbird gene and introduce it into bacterial plasmids.
Answer
Isolate gene -> Cut plasmid & gene -> Insert gene -> Seal plasmid -> Transform bacteria -> Select bacteria
The final answer highlights the cloning of genes in bacterial plasmids involves isolating the gene, cutting plasmids and gene with restriction enzymes, inserting the gene into the plasmid, sealing the recombinant plasmid with DNA ligase, transforming the plasmid into bacteria, and selecting transformed bacteria.
Answer for screen readers
The final answer highlights the cloning of genes in bacterial plasmids involves isolating the gene, cutting plasmids and gene with restriction enzymes, inserting the gene into the plasmid, sealing the recombinant plasmid with DNA ligase, transforming the plasmid into bacteria, and selecting transformed bacteria.
More Information
Gene cloning in plasmids is crucial for producing proteins, studying gene functions, and developing genetically modified organisms.
Tips
Common mistakes include not ensuring the gene and plasmid are cut with the same restriction enzymes, and incorrect selection conditions for modified bacteria.
Sources
- Overview: DNA cloning (article) - Khan Academy - khanacademy.org
- DNA cloning, sequencing, and analysis | NCBI Bookshelf - ncbi.nlm.nih.gov
- Traditional Cloning Basics | Thermo Fisher Scientific - thermofisher.com
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