What is Lishman's stain and what does it consist of?
Understand the Problem
The question involves understanding Lishman's stain, which is used for the examination of erythrocytes and the characteristics of various stains mentioned. Specifically, it lists components of Lishman's stain and describes their uses and chemical properties.
Answer
Eosin, methylene blue, acetone-free methanol.
Leishman's stain consists of eosin (acidic and red for cytoplasmic granules) and methylene blue (basic and blue for nuclei). It uses acetone-free methanol as a solvent and fixative.
Answer for screen readers
Leishman's stain consists of eosin (acidic and red for cytoplasmic granules) and methylene blue (basic and blue for nuclei). It uses acetone-free methanol as a solvent and fixative.
More Information
Leishman's stain is used extensively in microscopy for differentiating different blood cells and organisms in blood smears.
Tips
Ensure methanol is acetone-free to avoid unwanted reactions.
Sources
- Leishman stain - Wikipedia - en.wikipedia.org
- Leishman Stain - ScienceDirect Topics - sciencedirect.com
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