What is a Lineweaver-Burk plot?
Understand the Problem
The question is asking for an explanation of the Lineweaver-Burk plot, which is a graphical representation used in enzyme kinetics to depict the relationship between enzyme activity and substrate concentration. It involves plotting the reciprocal of the enzyme reaction rate versus the reciprocal of the substrate concentration to determine kinetic parameters.
Answer
A Lineweaver–Burk plot is a double reciprocal plot used to determine enzyme kinetics, specifically Km and Vmax.
The Lineweaver–Burk plot is a graphical representation of the double reciprocal of the Michaelis-Menten equation used to determine enzyme kinetics, specifically Km and Vmax.
Answer for screen readers
The Lineweaver–Burk plot is a graphical representation of the double reciprocal of the Michaelis-Menten equation used to determine enzyme kinetics, specifically Km and Vmax.
More Information
The Lineweaver-Burk plot, also known as the double reciprocal plot, is used to illustrate the relationship between enzyme velocity and substrate concentration. By plotting 1/v (reaction velocity) against 1/[S] (substrate concentration), one can determine the values of Km (Michaelis constant) and Vmax (maximum velocity).
Tips
Common mistakes include misinterpreting the axes of the plot and not understanding the significance of the intercepts.
Sources
- Lineweaver–Burk plot - Wikipedia - en.wikipedia.org
- Lineweaver-Burk Plots – MCAT Biochemistry - MedSchoolCoach - medschoolcoach.com
- 4.10: Lineweaver-Burk Plots - Biology LibreTexts - bio.libretexts.org
