What cannot be determined using the Electrophoretic Mobility Shift Assay (EMSA)? A) The binding affinity of protein-DNA interactions. B) The identity of the bound protein complex.... What cannot be determined using the Electrophoretic Mobility Shift Assay (EMSA)? A) The binding affinity of protein-DNA interactions. B) The identity of the bound protein complex. C) The size of the protein-DNA complex. D) The sequence of the radiolabeled DNA probe.
Understand the Problem
The question is asking which option among the given choices cannot be determined through the use of the Electrophoretic Mobility Shift Assay (EMSA). The options include aspects related to protein-DNA interactions such as binding affinity, identity of the protein complex, size of the complex, and the sequence of the DNA probe.
Answer
The EMSA cannot determine the sequence of the radiolabeled DNA probe.
The EMSA cannot determine the sequence of the radiolabeled DNA probe.
Answer for screen readers
The EMSA cannot determine the sequence of the radiolabeled DNA probe.
More Information
The EMSA is used to study protein-DNA interactions by observing shifts in mobility on a gel. While it can give insights into the presence and affinity of binding complexes, it does not provide information on the exact DNA sequence involved.
Tips
One common mistake is assuming EMSA can provide detailed sequence information. It's important to understand that EMSA results focus on binding dynamics, not on sequence elucidation.
Sources
- Electrophoretic Mobility Shift Assay - an overview - ScienceDirect.com - sciencedirect.com
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