isolation of rizobium from root nodules
Understand the Problem
The question is asking about the process of isolating Rhizobium bacteria from root nodules of leguminous plants. This involves understanding the methods used in microbiology to extract and culture these specific bacteria for study or agricultural purposes.
Answer
Surface sterilize, crush preserved nodules, spread on YEMAC medium, incubate for 4-5 days.
The final answer is Rhizobium can be isolated from root nodules by surface sterilizing preserved nodules, crushing them, and spreading the content over YEMAC medium; colonies form after 4-5 days.
Answer for screen readers
The final answer is Rhizobium can be isolated from root nodules by surface sterilizing preserved nodules, crushing them, and spreading the content over YEMAC medium; colonies form after 4-5 days.
More Information
The process involves the use of YEMAC medium, a commonly used medium for culturing microorganisms like Rhizobium. Proper surface sterilization is key to avoid contamination from other bacteria.
Tips
A common mistake is inadequate surface sterilization, which can lead to contamination by other bacteria.
Sources
- Isolation of Rhizobium from root nodule - nopr.niscpr.res.in
- A rapid method for the isolation and identification of Rhizobium from... - handelsmanlab.discovery.wisc.edu
AI-generated content may contain errors. Please verify critical information
