In an enzyme-linked immunosorbent assay (ELISA), how is the signal generated?
Understand the Problem
The question is asking about the mechanism of signal generation in an enzyme-linked immunosorbent assay (ELISA), which is a laboratory technique used for detecting and quantifying proteins, antibodies, and hormones. It involves understanding the principles and processes involved in ELISA.
Answer
A detectable signal, most commonly a color change.
The final answer is a detectable signal, most commonly a color change, generated by the reaction between the enzyme linked to an antibody and its substrate.
Answer for screen readers
The final answer is a detectable signal, most commonly a color change, generated by the reaction between the enzyme linked to an antibody and its substrate.
More Information
In ELISA, the enzyme conjugated to an antibody interacts with a substrate to yield a detectable signal. This signal is often a color change but can also be chemiluminescent or fluorometric depending on the type of substrate used.
Tips
Common mistakes include not thoroughly washing to remove unbound antibodies, which can lead to high background signals, and not using the correct substrate for the enzymatic reaction.
Sources
- Enzyme Linked Immunosorbent Assay - StatPearls - NCBI Bookshelf - ncbi.nlm.nih.gov
- Enzyme-linked immunosorbent assay (ELISA) - Wikipedia - en.wikipedia.org
- Overview of ELISA | Thermo Fisher Scientific - US - thermofisher.com
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