Explain why you cannot use a light microscope to view inside a subcellular structure. Describe simply how a TEM works. State and explain which microscope should be used to observe... Explain why you cannot use a light microscope to view inside a subcellular structure. Describe simply how a TEM works. State and explain which microscope should be used to observe the heartbeat of a Daphnia (water flea). A light microscope has a resolution of 2 x 10^-7 m, compared to 1 x 10^-10 m for a modern TEM. State how many times smaller an object can be viewed with the TEM than can be viewed with the light microscope.
Understand the Problem
The question is asking to explain why a light microscope cannot be used to view inside a subcellular structure, describe how a Transmission Electron Microscope (TEM) works, identify which microscope should be used to observe a specific organism, and compare the resolutions of light and electron microscopes. This involves knowledge of microscopy and cellular biology.
Answer
Light microscopes lack resolution for subcellular structures; TEMs use electrons for higher resolution but are unsuitable for live specimens. Use a light microscope for Daphnia. TEMs are 2,000 times more resolving than light microscopes.
- Light microscopes can't view inside subcellular structures because their resolution (200 nm) is insufficient for seeing smaller details. 2. TEMs use electron beams to achieve high resolution, passing electrons through thin samples to create detailed images. 3. Use a light microscope to observe a Daphnia's heartbeat since it allows for the observation of living organisms. 4. An object can be viewed 2,000 times smaller with a TEM (0.1 nm) than with a light microscope (200 nm).
Answer for screen readers
- Light microscopes can't view inside subcellular structures because their resolution (200 nm) is insufficient for seeing smaller details. 2. TEMs use electron beams to achieve high resolution, passing electrons through thin samples to create detailed images. 3. Use a light microscope to observe a Daphnia's heartbeat since it allows for the observation of living organisms. 4. An object can be viewed 2,000 times smaller with a TEM (0.1 nm) than with a light microscope (200 nm).
More Information
TEMs offer much higher resolution by using electron beams instead of light, but they require non-living samples, which isn’t suitable for observing live organisms like Daphnia.
Tips
Avoid using electron microscopes for live specimens as the process may be harmful.
Sources
- Microscopy: Intro to microscopes & how they work - khanacademy.org
- Why wouldn't an electron microscope be used to watch the heart of a Daphnia? - quora.com
- Light Microscope vs. Electron Microscope - excedr.com
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