Calculate the net charge of a peptide.
Understand the Problem
The question is asking for the calculation of the net charge of a peptide. This involves understanding the ionizable groups present in the peptide and their pKa values, as well as the pH of the solution in which the peptide is dissolved. The net charge can be determined by considering the ionization states of the amino acid side chains and termini at a given pH.
Answer
Sum the charges from all ionizable groups.
The final answer is to sum the charges from all ionizable groups.
Answer for screen readers
The final answer is to sum the charges from all ionizable groups.
More Information
To find the net charge of a peptide at a specific pH, it is important to know the pKa values of each ionizable group, allowing for accurate calculation of their charges at that pH.
Tips
A common mistake is forgetting to include the charges of the N-terminal amino group and the C-terminal carboxyl group when calculating the net charge of the peptide.
Sources
- Acid-Base Chemistry of Peptides - webhost.bridgew.edu
- Peptide Calculator & Amino Acid Calculator - Biosynth - biosynth.com
- Calculating Net Charge on Proteins - AK Lectures - aklectures.com
