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Questions and Answers
What is the ideal blood volume donation from a healthy donor animal?
What is the ideal blood volume donation from a healthy donor animal?
- No more than 5%
- No more than 10% (correct)
- No more than 20%
- No more than 15%
Which blood type is considered the universal donor for dogs?
Which blood type is considered the universal donor for dogs?
- DEA 1 positive
- DEA 1 negative (correct)
- Type A
- Type B
What maximum blood volume loss can a healthy donor animal handle without approaching hypovolemic shock?
What maximum blood volume loss can a healthy donor animal handle without approaching hypovolemic shock?
- 40%
- 30% (correct)
- 20%
- 10%
What is the main benefit of testing for pathogens like Anaplasma and Babesia before transfusion?
What is the main benefit of testing for pathogens like Anaplasma and Babesia before transfusion?
Why are male horses preferred as donors?
Why are male horses preferred as donors?
Which of the following is a common transfusion trigger for utilizing RBC products?
Which of the following is a common transfusion trigger for utilizing RBC products?
Donkeys and mules should not be used as blood donors for what reason?
Donkeys and mules should not be used as blood donors for what reason?
What is an important characteristic of prospective blood donors?
What is an important characteristic of prospective blood donors?
What hematocrit level is considered safe in otherwise healthy normovolemic patients?
What hematocrit level is considered safe in otherwise healthy normovolemic patients?
Which of the following is NOT a sign of anemia that should prompt immediate attention?
Which of the following is NOT a sign of anemia that should prompt immediate attention?
Why might patients with chronic anemia not require a transfusion despite low hematocrit levels?
Why might patients with chronic anemia not require a transfusion despite low hematocrit levels?
Which condition is a strong trigger for plasma transfusion?
Which condition is a strong trigger for plasma transfusion?
What is the most important canine blood type antigen?
What is the most important canine blood type antigen?
What defines DEA 1 positive dogs in the context of blood transfusion?
What defines DEA 1 positive dogs in the context of blood transfusion?
What is a major reason acute anemias may require transfusions at higher PCV levels than chronic anemias?
What is a major reason acute anemias may require transfusions at higher PCV levels than chronic anemias?
What does a rightward shift in the oxy-hemoglobin dissociation curve indicate?
What does a rightward shift in the oxy-hemoglobin dissociation curve indicate?
What type of blood can positive dogs receive?
What type of blood can positive dogs receive?
Why is blood typing critical for cats before a transfusion?
Why is blood typing critical for cats before a transfusion?
What is the minimum requirement in emergencies for feline blood transfusions if blood typing is not available?
What is the minimum requirement in emergencies for feline blood transfusions if blood typing is not available?
Which animal requires a cross match to assess compatibility but is primarily used for blood transfusions?
Which animal requires a cross match to assess compatibility but is primarily used for blood transfusions?
What is the main purpose of performing a major crossmatch?
What is the main purpose of performing a major crossmatch?
What happens to dogs that have been transfused longer than 4-5 days regarding transfusions?
What happens to dogs that have been transfused longer than 4-5 days regarding transfusions?
Which of the following blood types is crucial for dogs?
Which of the following blood types is crucial for dogs?
Why is cross matching primarily utilized in horses during blood transfusions?
Why is cross matching primarily utilized in horses during blood transfusions?
What is the maximum storage life of red blood cells in CPDA-1 anticoagulant?
What is the maximum storage life of red blood cells in CPDA-1 anticoagulant?
Which blood product contains active platelets and coagulation factors?
Which blood product contains active platelets and coagulation factors?
What is the primary purpose of component therapy in transfusion medicine?
What is the primary purpose of component therapy in transfusion medicine?
What is the indication for using fresh whole blood in animals?
What is the indication for using fresh whole blood in animals?
What is a consequence of administering stored whole blood greater than 6 hours old?
What is a consequence of administering stored whole blood greater than 6 hours old?
Which product is most suitable for treating chronic hemolytic anemia?
Which product is most suitable for treating chronic hemolytic anemia?
What condition might indicate the use of packed red blood cells?
What condition might indicate the use of packed red blood cells?
What is the typical starting dose of blood for transfusion in animals?
What is the typical starting dose of blood for transfusion in animals?
What does a crossmatch grade of 2+ indicate?
What does a crossmatch grade of 2+ indicate?
When evaluating blood for compatibility, which type of crossmatch is prioritized?
When evaluating blood for compatibility, which type of crossmatch is prioritized?
What is the main reason for performing an autoagglutination screen?
What is the main reason for performing an autoagglutination screen?
What is true about the presence of incompatible antibodies in a major crossmatch reaction?
What is true about the presence of incompatible antibodies in a major crossmatch reaction?
Which of the following statements regarding blood product administration is correct?
Which of the following statements regarding blood product administration is correct?
What is the significance of having a higher amount of plasma in the recipient compared to donor RBCs?
What is the significance of having a higher amount of plasma in the recipient compared to donor RBCs?
What does a crossmatch grade of 0 signify?
What does a crossmatch grade of 0 signify?
How should pRBCs be modified before administration if needed?
How should pRBCs be modified before administration if needed?
What can A-B incompatibility in cats lead to?
What can A-B incompatibility in cats lead to?
What is the recommended practice for cats due to the Mik antigen?
What is the recommended practice for cats due to the Mik antigen?
Which blood group systems are predominant in horses?
Which blood group systems are predominant in horses?
What happens when foals inherit a different blood type from their mare?
What happens when foals inherit a different blood type from their mare?
What is the role of blood typing prior to transfusion in dogs and cats?
What is the role of blood typing prior to transfusion in dogs and cats?
Which method is commonly used for blood typing in practice?
Which method is commonly used for blood typing in practice?
What should DEA 1 negative dogs receive?
What should DEA 1 negative dogs receive?
What is a key consideration when typing blood?
What is a key consideration when typing blood?
Flashcards
Ideal blood donation percentage
Ideal blood donation percentage
The ideal blood volume to donate from a healthy donor is only 10% of their total blood volume.
Horse donor antigen screening
Horse donor antigen screening
Donors for horses should be tested for certain specific antigens, like Qa and Aa. They should also be negative for these antigens and their antibodies.
Donkey factor free plasma
Donkey factor free plasma
Donors for horses should be certified as 'donkey factor free' if their plasma is used to treat failure of passive transfer in donkeys or mules. This ensures the plasma doesn't contain antibodies that could harm the foal.
Cat blood type screening
Cat blood type screening
Donors for cats should be tested for both A and B blood types because cats need type-specific transfusions.
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Ideal blood donor characteristics
Ideal blood donor characteristics
The ideal blood donor is a young, healthy animal that has never received a blood transfusion. They should be screened for various health indicators and potential pathogens.
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Dog donor pathogen screening
Dog donor pathogen screening
Dogs should be screened for specific pathogens like Anaplasma, Babesia, Bartonella, Ehrlichia, Leishmania, and Mycoplasma. This helps prevent transmitting infections to the recipient dog.
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DEA 1 negative dog donor
DEA 1 negative dog donor
Ideally, dog blood donors are DEA 1 negative, making them 'universal donors' as their blood can be given to most dogs.
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Maximum blood donation volume
Maximum blood donation volume
A large blood donation, up to 20% of the donor's blood volume, can be tolerated by a healthy donor. However, this is considered the maximum donation volume.
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Anemia
Anemia
A condition where the body's ability to carry oxygen is reduced due to a low red blood cell count.
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Hematocrit (HCT)
Hematocrit (HCT)
The percentage of red blood cells in the blood.
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Red Blood Cell Transfusion
Red Blood Cell Transfusion
A type of blood transfusion where only the red blood cells are given to the patient.
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Plasma Transfusion
Plasma Transfusion
A type of blood transfusion where only the liquid component of blood is given to the patient.
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Blood Typing
Blood Typing
The process of identifying the blood type of an animal.
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DEA 1 Antigen
DEA 1 Antigen
Antigen found on the surface of red blood cells in dogs. The presence or absence of this antigen determines a dog's blood type.
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Immune Response to DEA 1 Positive Blood
Immune Response to DEA 1 Positive Blood
The immune response that occurs when a dog with the DEA 1 negative blood type receives blood from a dog with the DEA 1 positive blood type.
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Coagulopathy
Coagulopathy
A condition where the body's ability to produce blood clotting factors is impaired.
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Neonatal Isoerythrolysis in Cats
Neonatal Isoerythrolysis in Cats
A type of blood incompatibility in cats where kittens receiving colostrum from a mother with a different blood type can suffer from hemolysis.
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Mik Antigen
Mik Antigen
An additional red blood cell antigen found in most cats that can cause hemolytic transfusion reactions if a cat lacking the Mik antigen receives blood from a Mik antigen-positive donor.
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Equine Blood Types
Equine Blood Types
A blood type system in horses with over 30 red blood cell factors, with the Ca antigen being particularly important.
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Neonatal Isoerythrolysis (NI) in Horses
Neonatal Isoerythrolysis (NI) in Horses
A condition in foals where the blood type of the mare and foal differ, and the foal inherits the stallion's sensitizing red blood cell type, leading to hemolysis.
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DEA 1 Negative Dogs
DEA 1 Negative Dogs
A negative blood type in dogs that allows dogs with this blood type to be universal donors.
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Card Type Blood Typing
Card Type Blood Typing
A blood typing method that uses cards or tubes with specific antibodies that react with the patient's blood to determine their blood type.
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Immunochromatography Blood Typing
Immunochromatography Blood Typing
A blood typing method that uses a strip with antibodies that react with the patient's blood to determine their blood type.
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Autoagglutination
Autoagglutination
A situation where a patient's blood cells clump together due to an immune response.
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Major crossmatch
Major crossmatch
A test that identifies the presence of antibodies in recipient plasma that react with donor red blood cells.
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Minor crossmatch
Minor crossmatch
A test that identifies the presence of antibodies in recipient red blood cells that react with donor plasma.
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Crossmatch Grade 0
Crossmatch Grade 0
A crossmatch grade indicating no agglutination (clumping) of red blood cells.
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Crossmatch Grade 2+
Crossmatch Grade 2+
A crossmatch grade indicating a few large agglutinates (clumps) mixed with smaller clumps of red blood cells.
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Autoagglutination Screen
Autoagglutination Screen
A test to identify autoagglutination in recipient blood, which can hinder the accuracy of crossmatch results.
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Major Incompatibility
Major Incompatibility
Incompatibility between donor and recipient blood that can lead to serious reactions during transfusion.
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Minor Incompatibility
Minor Incompatibility
Incompatibility between donor and recipient blood that can lead to a mild reaction during transfusion.
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Ideal Crossmatch
Ideal Crossmatch
A type of crossmatch where the donor blood chosen is compatible by both major and minor crossmatch.
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What are universal donor dogs?
What are universal donor dogs?
Dogs with DEA 1 negative blood are considered "universal donors" because they lack the DEA 1 antigen. This allows their blood to be safely transfused into most dogs without triggering a strong immune response.
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Why is cat blood typing essential?
Why is cat blood typing essential?
Cats must be blood typed before transfusion because giving the wrong type can be fatal. This is due to the presence of A and B blood types, which can lead to a severe immune reaction when incompatible types are mixed.
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What happens in a major crossmatch?
What happens in a major crossmatch?
A major crossmatch test involves mixing the donor's red blood cells with the recipient's plasma. This helps identify any antibodies in the recipient's plasma that could attack the donor's red blood cells during transfusion.
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What is the purpose of crossmatching?
What is the purpose of crossmatching?
Crossmatching is a crucial test to assess blood compatibility before a transfusion. It helps detect antibodies in the recipient's or donor's plasma that could lead to a transfusion reaction.
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What is given in a dog emergency if their blood type is unknown?
What is given in a dog emergency if their blood type is unknown?
In an emergency, when a dog's blood type is unknown, DEA 1-negative blood can be given as a universal donor. This is because most dogs don't have antibodies against the DEA 1 antigen, minimizing the risk of a transfusion reaction.
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What happens in a minor crossmatch?
What happens in a minor crossmatch?
A minor crossmatch mixes donor plasma with recipient red blood cells. It aims to detect any antibodies in the donor's plasma that could potentially react with the recipient's blood cells.
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What are the limitations of crossmatching?
What are the limitations of crossmatching?
While crossmatching helps assess compatibility, it doesn't eliminate the possibility of transfusion reactions. It also doesn't prevent future sensitization to antigens, which could cause problems in future transfusions.
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When are dogs retested for blood typing?
When are dogs retested for blood typing?
Dogs that have received a transfusion within 4-5 days should be typed and crossmatched. This is because it takes time for the body to develop antibodies against antigens following a transfusion.
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Packed Red Blood Cells (pRBCs)
Packed Red Blood Cells (pRBCs)
A blood product containing only red blood cells and a small amount of plasma, used to treat various anemias and reduce the risk of volume overload.
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Fresh Whole Blood
Fresh Whole Blood
A blood product that is administered within 6 hours of donation, containing all coagulation factors, active platelets, and plasma proteins, suitable for acute hemorrhage and anemic patients with coagulopathy.
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Stored Whole Blood
Stored Whole Blood
Whole blood that has been stored for more than 6 hours, lacking active platelets and some coagulation factors, used for acute hemorrhage in animals with coagulopathy or hypoproteinemia.
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Component Therapy
Component Therapy
A method of using individual blood components instead of whole blood, minimizing the risk of antigen exposure and maximizing the number of patients treated per donation.
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Red Cell Extenders
Red Cell Extenders
A type of blood product used to enhance red cell storage life to 42 days by preventing cell damage.
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Major Learning Goals: VETM 5291: Transfusion Medicine
- List characteristics of a good blood donor and calculate maximum donation volume.
- List red blood cell transfusion triggers in dogs, cats, and horses.
- Identify important blood groups in dogs, cats, and horses and how they affect transfusion decisions.
- Interpret blood typing tests for dogs and cats.
- Understand indications for crossmatching blood (major and minor crossmatch) and interpretation of crossmatch results.
- Identify different types of transfusion reactions and strategies for avoidance and monitoring.
Blood Products
- Blood products are used to treat anemia, coagulopathy, and hypoproteinemia.
- Common products include fresh whole blood, packed red blood cells (pRBCs), and plasma.
- Product selection depends on the animal's needs (e.g., RBCs for anemia, fresh whole blood for multiple needs).
- Blood banking is well developed for dogs and cats, with readily available products.
- Local donors are often used for animals requiring infrequent transfusions.
Donor Characteristics
- Ideal donors are healthy, young, fit animals that haven't received previous transfusions.
- Potential donors should be screened for health, blood type, and potential pathogens.
- Dogs: DEA 1 negative are "universal donors."
- Cats: A and B type donors are needed, as they must receive type specific blood.
Recipient Characteristics
- Transfusion triggers depend on patient circumstances.
- Oxygen delivery to tissues is maximized in hematocrits of 30-40%.
- Low hematocrits (as low as 18%) are safe in healthy normovolemic patients.
- Clinicians should consider ongoing blood loss, chronicity of anemia, and other concurrent illness.
- Patients with acute anemia (e.g., IMHA, hemorrhage) require higher PCV transfusions than chronic anemia patients.
Blood Typing
- Canine Blood Types: DEA 1 antigen is the most significant.
- Majority of dogs are DEA 1 positive.
- DEA 1 negative dogs are considered "universal donors.".
- Testing for other antigens (DEA 3, 4, 5, 6, 7, and 8, Kai1, Kai 2, and Dal antigen) is also important, but not commonly routinely tested.
- Feline Blood Types: Cats have A, B, or AB types with type A being overwhelmingly common.
- Type B cats have naturally occurring anti-A antibodies.
- Type A cats may have weak anti-B antibodies.
- A-B mismatches result in immediate, potentially fatal reaction.
Crossmatching
- Crossmatching detects anti-RBC antibodies in donor or recipient plasma.
- Essential in determining compatibility between donor and recipient blood.
- Major and minor crossmatches assess donor RBCs with recipient plasma and vice versa.
- Grades of agglutination in crossmatches are used to classify compatibility.
- Ideal match is no agglutination (grade 0).
Blood Product Administration and Monitoring
- Administration of blood should be slow, with thorough monitoring to identify any reactions.
- Reactions (e.g., hemolytic, allergic, febrile) may occur.
- Proper handling and storage of blood products is crucial to avoid bacterial contamination and product degradation.
- Monitor for signs of transfusion reactions (e.g., fever, dyspnea, hypotension).
- Take baseline vital signs and re-evaluate periodically.
Transfusion Reactions
- Four major classes: acute immunologic, delayed immunologic, acute non-immunologic, and delayed non-immunologic.
- Acute immunologic: Antibodies and complement attack donor RBCs causing hemolysis (e.g., fever, hypotension, hemoglobinuria).
- Delayed immunologic: Immune-mediated reactions to blood factors (e.g., previous sensitization may occur).
- Acute non-immunologic: Reactions related to handling, administration, or metabolic changes (e.g., febrile reactions, circulatory overload).
- Delayed non-immunologic: Infections or blood product complications (e.g., blood-borne infections).
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