Practical Medical Microbiology Lab Sterilization and Disinfection Quiz

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Questions and Answers

What is the main difference between sterilization and disinfection?

  • Sterilization and disinfection both use chemical solutions to eliminate most pathogenic microorganisms, but sterilization also kills bacterial spores
  • Sterilization only eliminates most pathogenic microorganisms but not bacterial spores, while disinfection kills all microorganisms, including bacterial spores
  • Sterilization and disinfection both kill all microorganisms, including bacterial spores, but sterilization is achieved by physical means and disinfection is achieved by chemical means
  • Sterilization kills all microorganisms, including bacterial spores, while disinfection eliminates most pathogenic microorganisms but not bacterial spores (correct)

Which of the following is an example of asepsis?

  • Applying antiseptics topically on animate surfaces
  • Lowering microbial counts in public settings to prevent transmission
  • Using bacteriocidal agents on inanimate objects
  • Absence of significant contamination (correct)

What distinguishes bacteriocidal agents from bacteriostatic agents?

  • Bacteriocidal agents and bacteriostatic agents both kill bacteria, including endospores, but bacteriocidal agents also inhibit the growth of bacteria
  • Bacteriocidal agents and bacteriostatic agents are both used to prevent infection on animate surfaces
  • Bacteriocidal agents only inhibit the growth of bacteria, while bacteriostatic agents kill bacteria, including endospores
  • Bacteriocidal agents kill bacteria, including endospores, while bacteriostatic agents inhibit the growth of bacteria but do not necessarily kill them (correct)

What does sepsis indicate in microbiology?

<p>Bacterial contamination (A)</p> Signup and view all the answers

What is the purpose of sanitization in public settings?

<p>To prevent transmission by lowering microbial counts to a safe level (A)</p> Signup and view all the answers

Which statement best describes antiseptics?

<p>Agents that prevent infection by inhibiting the growth of microorganisms and can be applied topically on animate surfaces (D)</p> Signup and view all the answers

Which method is responsible for spontaneous sterilization in natural conditions?

<p>Sunlight (A)</p> Signup and view all the answers

Which method is unreliable for sterilizing spores?

<p>Drying (B)</p> Signup and view all the answers

What is the main purpose of filtration in microbiology?

<p>Removing microorganisms from heat-sensitive solutions (A)</p> Signup and view all the answers

What type of radiation has greater capacity to induce lethal changes in the cell's genome (DNA)?

<p>Ionizing rays (C)</p> Signup and view all the answers

Which dry heat method includes sterilization using a Bunsen flame till the article becomes red hot?

<p>Red heat (C)</p> Signup and view all the answers

At what temperature is the holding method employed for pasteurization in the food and dairy industry?

<p>$63C°$ (A)</p> Signup and view all the answers

Which method is used to sterilize all glassware at high temperatures?

<p>$180C°$/30 min (B)</p> Signup and view all the answers

What is the main action of moist heat on microorganisms?

<p>Denaturation of proteins (B)</p> Signup and view all the answers

Which type of filtration is used for removing microorganisms from air?

<p>Filtration using high efficiency particulate air filters (C)</p> Signup and view all the answers

What is the most common and excellent method for treating infectious waste in hospitals?

<p>Incineration (B)</p> Signup and view all the answers

Flashcards

Sterilization

The process of killing all microorganisms, including bacterial spores, using methods like heat, radiation, or chemicals.

Disinfection

The process of reducing the number of pathogenic microorganisms to a safe level, often using chemical disinfectants.

Asepsis

The absence of significant contamination by microorganisms, often achieved through techniques like sterile equipment and practices.

Bactericidal agents

Agents that kill bacteria, including their resistant endospores, effectively eliminating bacterial populations.

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Bacteriostatic agents

Agents that inhibit the growth of bacteria, preventing them from multiplying but not necessarily killing them.

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Sepsis

The presence of bacteria in the blood or other tissues, often leading to systemic infection and complications.

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Sanitization

The process of reducing the number of microorganisms in a public setting to a safe level, often using chemicals or cleaning methods.

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Antiseptics

Agents applied topically to living surfaces to prevent infection by inhibiting the growth of microorganisms.

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Sunlight sterilization

The natural process of sterilization by the ultraviolet rays of sunlight, effectively killing microorganisms.

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Filtration

A method of sterilization that focuses on removing microorganisms from heat-sensitive solutions using filters with pore sizes small enough to trap microbes.

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Ionizing radiation

A type of radiation, like X-rays or gamma rays, that carries enough energy to damage DNA and kill microorganisms.

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Red heat sterilization

A dry heat method of sterilization that involves heating an instrument in a Bunsen flame until it glows red hot, killing all microorganisms.

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Holding method pasteurization

A method of pasteurization used in the food and dairy industry that involves heating the product at 63°C for a specific duration to kill harmful microorganisms.

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Moist heat sterilization for glassware

A method of sterilization using moist heat, typically involving heating glassware at 180°C for 30 minutes to ensure complete sterilization.

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Protein denaturation by moist heat

The main mechanism by which moist heat sterilizes microorganisms, by breaking down the proteins essential for microbial survival and function.

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HEPA filtration

The process of removing microorganisms from air using specialized filters known as HEPA filters, which capture particles with remarkable efficiency.

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Incineration of infectious waste

The most commonly used and highly effective method for treating infectious waste in hospitals. It involves burning the waste at high temperatures, completely eliminating all microorganisms and making it safe.

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