Medical Microbiology and Immunology Practice: Physical Methods of Sterilization and Disinfection
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Questions and Answers

Which term refers to the removal of many or all pathogenic organisms, usually not including spores?

  • Disinfection (correct)
  • Sterilization
  • Antiseptic
  • Inoculation

    • What is the main purpose of sterilization?

  • To prevent the growth of microorganisms
  • To kill all microorganisms, including spores (correct)
  • To remove pathogenic organisms
  • To reduce the number of microorganisms

    • Which physical method of sterilization involves the use of infra-red rays?

  • Chemical
  • Dry heat
  • Filtration
  • Irradiation (correct)

    • What is the primary purpose of disinfectants?

    <p>To remove many or all pathogenic organisms</p> Signup and view all the answers

    What is the main difference between dry heat and moist heat sterilization methods?

    <p>Effectiveness against spores</p> Signup and view all the answers

    Which physical method of sterilization involves the use of a hot air oven?

    <p>Dry heat</p> Signup and view all the answers

    What is the holding time for sterilization in a hot air oven at 180°C?

    <p>1 hour</p> Signup and view all the answers

    Which precaution is recommended for sterilizing glass in a hot air oven?

    <p>Putting the glass while the oven is already hot</p> Signup and view all the answers

    At what temperature is pasteurization of milk typically carried out?

    <p>63°C</p> Signup and view all the answers

    What is the purpose of using Browne’s tubes in monitoring the sterilization process in a hot air oven?

    <p>To indicate successful sterilization by changing color</p> Signup and view all the answers

    What is the temperature and holding time required for unwrapped instruments in an autoclave for sterilization?

    <p>$121°C$ for 20 min</p> Signup and view all the answers

    What is the purpose of using 2% NaHCO3 in boiling for sterilization?

    <p>To promote sterilization and prevent rusting</p> Signup and view all the answers

    What is the principle behind steaming (Koch’s steamer) at 100°C for sterilization?

    <p>Heating under pressure to increase steam temperature</p> Signup and view all the answers

    What is the primary purpose of sterilization by filtration?

    <p>To remove bacteria from fluids through filters</p> Signup and view all the answers

    What is the main difference between sterilization by dry heat and moist heat?

    <p>Dry heat involves higher temperatures than moist heat.</p> Signup and view all the answers

    What is the purpose of using Bacillus atrophaeus spores in biological controls for monitoring dry heat sterilization?

    <p>To indicate successful sterilization by changing color</p> Signup and view all the answers

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