Medical Microbiology and Immunology Practice: Physical Methods of Sterilization and Disinfection
16 Questions
4 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Which term refers to the removal of many or all pathogenic organisms, usually not including spores?

  • Disinfection (correct)
  • Sterilization
  • Antiseptic
  • Inoculation

What is the main purpose of sterilization?

  • To prevent the growth of microorganisms
  • To kill all microorganisms, including spores (correct)
  • To remove pathogenic organisms
  • To reduce the number of microorganisms

Which physical method of sterilization involves the use of infra-red rays?

  • Chemical
  • Dry heat
  • Filtration
  • Irradiation (correct)

What is the primary purpose of disinfectants?

<p>To remove many or all pathogenic organisms (C)</p> Signup and view all the answers

What is the main difference between dry heat and moist heat sterilization methods?

<p>Effectiveness against spores (C)</p> Signup and view all the answers

Which physical method of sterilization involves the use of a hot air oven?

<p>Dry heat (C)</p> Signup and view all the answers

What is the holding time for sterilization in a hot air oven at 180°C?

<p>1 hour (C)</p> Signup and view all the answers

Which precaution is recommended for sterilizing glass in a hot air oven?

<p>Putting the glass while the oven is already hot (C)</p> Signup and view all the answers

At what temperature is pasteurization of milk typically carried out?

<p>63°C (C)</p> Signup and view all the answers

What is the purpose of using Browne’s tubes in monitoring the sterilization process in a hot air oven?

<p>To indicate successful sterilization by changing color (D)</p> Signup and view all the answers

What is the temperature and holding time required for unwrapped instruments in an autoclave for sterilization?

<p>$121°C$ for 20 min (A)</p> Signup and view all the answers

What is the purpose of using 2% NaHCO3 in boiling for sterilization?

<p>To promote sterilization and prevent rusting (C)</p> Signup and view all the answers

What is the principle behind steaming (Koch’s steamer) at 100°C for sterilization?

<p>Heating under pressure to increase steam temperature (B)</p> Signup and view all the answers

What is the primary purpose of sterilization by filtration?

<p>To remove bacteria from fluids through filters (D)</p> Signup and view all the answers

What is the main difference between sterilization by dry heat and moist heat?

<p>Dry heat involves higher temperatures than moist heat. (C)</p> Signup and view all the answers

What is the purpose of using Bacillus atrophaeus spores in biological controls for monitoring dry heat sterilization?

<p>To indicate successful sterilization by changing color (C)</p> Signup and view all the answers

Flashcards

Sterilization

A process designed to kill all microorganisms, including spores.

Disinfection

A process to remove many or all pathogenic organisms, but not necessarily spores.

Irradiation Sterilization

A method that utilizes infra-red rays to kill microorganisms.

Disinfectants

Chemicals used to remove or destroy pathogens on surfaces.

Signup and view all the flashcards

Dry Heat Sterilization

A method that uses hot air to kill microorganisms.

Signup and view all the flashcards

Moist Heat Sterilization

A sterilization method using heat and moisture to kill microorganisms.

Signup and view all the flashcards

Autoclave Sterilization

A method of sterilization using steam under pressure.

Signup and view all the flashcards

Pasteurization

The process of heating a liquid, typically milk, to a specific temperature for a set time to kill harmful microorganisms.

Signup and view all the flashcards

Hot Air Oven Sterilization

A sterilization method that uses a hot air oven to kill microorganisms by heating at a specific temperature for a set time.

Signup and view all the flashcards

Browne's Tube

A type of test tube used for monitoring the effectiveness of sterilization methods.

Signup and view all the flashcards

Koch's Steamer Sterilization

A special type of sterilization method using steam at 100°C.

Signup and view all the flashcards

Filtration Sterilization

A method of sterilization by physically filtering out microorganisms from fluids.

Signup and view all the flashcards

Bacillus atrophaeus Spore Test

Bacillus atrophaeus spores are used to test the effectiveness of dry heat sterilization.

Signup and view all the flashcards

Boiling Water Sterilization

A method of sterilization using boiling water, often with the addition of 2% NaHCO3 to enhance sterilization and minimize rusting.

Signup and view all the flashcards

Dry Heat vs. Moist Heat

The difference in effectiveness against spores.

Signup and view all the flashcards

Boiling Water Sterilization with NaHCO3

Using boiling water with the addition of 2% sodium bicarbonate (NaHCO3) to enhance sterilization and prevent rusting of the instruments.

Signup and view all the flashcards

More Like This

Use Quizgecko on...
Browser
Open
Browser
Browser