Medical Microbiology and Immunology Practice: Physical Methods of Sterilization and Disinfection

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Which term refers to the removal of many or all pathogenic organisms, usually not including spores?

Disinfection (correct)

Sterilization

Antiseptic

Inoculation

What is the main purpose of sterilization?

To prevent the growth of microorganisms

To kill all microorganisms, including spores (correct)

To remove pathogenic organisms

To reduce the number of microorganisms

Which physical method of sterilization involves the use of infra-red rays?

Chemical

Dry heat

Filtration

Irradiation (correct)

What is the primary purpose of disinfectants?

To remove many or all pathogenic organisms Signup and view all the answers

What is the main difference between dry heat and moist heat sterilization methods?

Effectiveness against spores Signup and view all the answers

Which physical method of sterilization involves the use of a hot air oven?

Dry heat Signup and view all the answers

What is the holding time for sterilization in a hot air oven at 180°C?

1 hour Signup and view all the answers

Which precaution is recommended for sterilizing glass in a hot air oven?

Putting the glass while the oven is already hot Signup and view all the answers

At what temperature is pasteurization of milk typically carried out?

63°C Signup and view all the answers

What is the purpose of using Browne’s tubes in monitoring the sterilization process in a hot air oven?

To indicate successful sterilization by changing color Signup and view all the answers

What is the temperature and holding time required for unwrapped instruments in an autoclave for sterilization?

$121°C$ for 20 min Signup and view all the answers

What is the purpose of using 2% NaHCO3 in boiling for sterilization?

To promote sterilization and prevent rusting Signup and view all the answers

What is the principle behind steaming (Koch’s steamer) at 100°C for sterilization?

Heating under pressure to increase steam temperature Signup and view all the answers

What is the primary purpose of sterilization by filtration?

To remove bacteria from fluids through filters Signup and view all the answers

What is the main difference between sterilization by dry heat and moist heat?

Dry heat involves higher temperatures than moist heat. Signup and view all the answers

What is the purpose of using Bacillus atrophaeus spores in biological controls for monitoring dry heat sterilization?

To indicate successful sterilization by changing color Signup and view all the answers

Medical Microbiology and Immunology Practice: Physical Methods of Sterilization and Disinfection

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